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In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

التفاصيل البيبلوغرافية
العنوان: In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis
المؤلفون: Paola Rosati, Martina Rossitto, Veronica Lupetti, Arianna Pompilio, Ersilia Fiscarelli, Giovanni Di Bonaventura, Valentina Crocetta, Nour Essa, Andrea Di Giulio
المساهمون: Vita Fiscarelli, E, Rossitto, M, Rosati, P, Essa, N, Crocetta, V, Di Giulio, A, Lupetti, V, Di Bonaventura, G, Pompilio, A
المصدر: Microorganisms
Volume 9
Issue 3
Microorganisms, Vol 9, Iss 478, p 478 (2021)
بيانات النشر: Multidisciplinary Digital Publishing Institute, 2021.
سنة النشر: 2021
مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), antibiotic resistance, medicine.drug_class, 030106 microbiology, Antibiotics, newly isolated environmental phage, Biology, medicine.disease_cause, Microbiology, Cystic fibrosis, Meropenem, biofilm, cystic fibrosis, 03 medical and health sciences, planktonic cells, Virology, medicine, Tobramycin, lcsh:QH301-705.5, Pseudomonas aeruginosa, chronic lung infection, Biofilm, bacteriophage in vitro activity, medicine.disease, biology.organism_classification, newly isolated environmental phages, chronic lung infections, 030104 developmental biology, lcsh:Biology (General), Amikacin, planktonic cell, phage efficacy, Bacteria, medicine.drug
الوصف: As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p <
0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.
وصف الملف: application/pdf
اللغة: English
تدمد: 2076-2607
DOI: 10.3390/microorganisms9030478
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17cd75b12ba266abbed917be7fab6119
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....17cd75b12ba266abbed917be7fab6119
قاعدة البيانات: OpenAIRE
الوصف
تدمد:20762607
DOI:10.3390/microorganisms9030478