Genetic Variation of Bordetella pertussis in Austria
| العنوان: | Genetic Variation of Bordetella pertussis in Austria |
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| المؤلفون: | Maria Paulke-Korinek, Pamela Rendi-Wagner, Helmut Mittermayer, Sabine Stumvoll, Frits R. Mooi, Harald H. Kessler, Gerold Stanek, Ralf Steinborn, Michael Duchêne, Birgit Wagner, Georg Freymüller, Helen Melzer, Herwig Kollaritsch, Andreas Repa, Ursula Wiedermann |
| المصدر: | PLoS One, 10 PLoS ONE PLoS One, 10, 7 PLoS ONE, Vol 10, Iss 7, p e0132623 (2015) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Bordetella pertussis, Whooping Cough, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], lcsh:Medicine, Gene Expression, Nasopharynx, Genotype, Virulence Factors, Bordetella, lcsh:Science, Child, Pertussis Vaccine, Multidisciplinary, biology, Vaccination, Bacterial Typing Techniques, Austria, Child, Preschool, Female, Fimbriae Proteins, Pertactin, medicine.drug, Research Article, Adult, DNA, Bacterial, Adolescent, Molecular Sequence Data, Multiple Loci VNTR Analysis, Pertussis toxin, Microbiology, Bacterial Proteins, medicine, Humans, Whooping cough, Polymorphism, Genetic, Base Sequence, Immunization Programs, lcsh:R, Infant, Newborn, Infant, biology.organism_classification, medicine.disease, Virology, Protein Subunits, Pertussis Toxin, Multilocus sequence typing, Pertussis vaccine, lcsh:Q, Multilocus Sequence Typing, Transcription Factors |
| الوصف: | Contains fulltext : 155346.PDF (Publisher’s version ) (Open Access) In Austria, vaccination coverage against Bordetella pertussis infections during infancy is estimated at around 90%. Within the last years, however, the number of pertussis cases has increased steadily, not only in children but also in adolescents and adults, indicating both insufficient herd immunity and vaccine coverage. Waning immunity in the host and/or adaptation of the bacterium to the immunised hosts could contribute to the observed re-emergence of pertussis. In this study we therefore addressed the genetic variability in B. pertussis strains from several Austrian cities. Between the years 2002 and 2008, 110 samples were collected from Vienna (n = 32), Linz (n = 63) and Graz (n = 15) by nasopharyngeal swabs. DNA was extracted from the swabs, and bacterial sequence polymorphisms were examined by MLVA (multiple-locus variable number of tandem repeat analysis) (n = 77), by PCR amplification and conventional Sanger sequencing of the polymorphic regions of the prn (pertactin) gene (n = 110), and by amplification refractory mutation system quantitative PCR (ARMS-qPCR) (n = 110) to directly address polymorphisms in the genes encoding two pertussis toxin subunits (ptxA and ptxB), a fimbrial adhesin (fimD), tracheal colonisation factor (tcfA), and the virulence sensor protein (bvgS). Finally, the ptxP promoter region was screened by ARMS-qPCR for the presence of the ptxP3 allele, which has been associated with elevated production of pertussis toxin. The MLVA analysis revealed the highest level of polymorphisms with an absence of MLVA Type 29, which is found outside Austria. Only Prn subtypes Prn1/7, Prn2 and Prn3 were found with a predominance of the non-vaccine type Prn2. The analysis of the ptxA, ptxB, fimD, tcfA and bvgS polymorphisms showed a genotype mixed between the vaccine strain Tohama I and a clinical isolate from 2006 (L517). The major part of the samples (93%) displayed the ptxP3 allele. The consequences for the vaccination strategy are discussed. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17fc9be60ae2c7de2d34ecdea4a93221 https://doi.org/10.1371/journal.pone.0132623 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....17fc9be60ae2c7de2d34ecdea4a93221 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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