Umbilical Cord Transforming Growth Factor-β3: Isolation, Comparison with Recombinant TGF-β3 and Cellular Localization
| العنوان: | Umbilical Cord Transforming Growth Factor-β3: Isolation, Comparison with Recombinant TGF-β3 and Cellular Localization |
|---|---|
| المؤلفون: | K K Iwata, G Y Qu, Brian T. Chait, David Fenyö, K Stam, Marley G, Ng Ay, Haley Jd, Daniel R. Marshak, A A Stewart |
| المصدر: | Growth Factors. 13:87-98 |
| بيانات النشر: | Informa UK Limited, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Gene isoform, TGF alpha, Blotting, Western, Molecular Sequence Data, Clinical Biochemistry, Umbilical Cord, law.invention, Endocrinology, Transforming Growth Factor beta, law, Humans, Amino Acid Sequence, Amino Acids, Cellular localization, Sequence Homology, Amino Acid, biology, Antibodies, Monoclonal, Cell Biology, Transforming growth factor beta, Immunohistochemistry, Molecular biology, Recombinant Proteins, Molecular Weight, Gene Expression Regulation, TGF-beta-3, Transforming growth factor, beta 3, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Mutagenesis, Site-Directed, biology.protein, Recombinant DNA, Electrophoresis, Polyacrylamide Gel, Sequence Analysis, Cell Division, Transforming growth factor |
| الوصف: | The transforming growth factor beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues. The TGF-beta 3 isoform was purified to homogeneity from both natural and recombinant sources and characterized by laser desorption mass spectrometry, by protein sequencing, by amino acid analysis and by biological activity. TGF-beta 3 was the major TGF-beta isoform in umbilical cord (230 ng/g), and was physically and biologically indistinguishable from recombinant TGF-beta 3 and from the tumor growth inhibitory (TGI) protein found in umbilical cord. Immunohistochemistry using antipeptide TGF-beta 3 specific antibody showed TGF-beta 3 localization in perivascular smooth muscle. |
| تدمد: | 1029-2292 0897-7194 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18444e46a95596c2059fd3597976b047 https://doi.org/10.3109/08977199609034569 |
| رقم الأكسشن: | edsair.doi.dedup.....18444e46a95596c2059fd3597976b047 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 10292292 08977194 |
|---|