IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
| العنوان: | IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells |
|---|---|
| المؤلفون: | Zhiguo Rao, Anran Zhang, Bi-Cheng Zhang, Xiaohua Li, Jian Liu, Yong Zhao, Dian-Chun Fang, Jian-Fei Gao, Yafei Zhang, Jie Zhao |
| المصدر: | Brazilian Journal of Medical and Biological Research v.46 n.1 2013 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Vol 46, Iss 1, Pp 32-38 (2013) Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 1, Pages: 32-38, Published: 28 SEP 2012 |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Physiology, Hepatocellular carcinoma, Stimulation, Biochemistry, Recurrence, General Pharmacology, Toxicology and Pharmaceutics, lcsh:QH301-705.5, Regulation of gene expression, lcsh:R5-920, General Neuroscience, Liver Neoplasms, Carcinoma prognosis, General Medicine, MiR-26a, Middle Aged, Immunohistochemistry, Up-Regulation, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, c-Myc, Hepatocyte, Female, lcsh:Medicine (General), Adult, Transcriptional Activation, medicine.medical_specialty, Carcinoma, Hepatocellular, Immunology, Biophysics, Ocean Engineering, Biology, Downregulation and upregulation, Internal medicine, medicine, Carcinoma, Humans, IL-6, Oncogene, Interleukin-6, Biomedical Sciences, Cell Biology, medicine.disease, MicroRNAs, Endocrinology, lcsh:Biology (General), Case-Control Studies, Cancer research |
| الوصف: | A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P < 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P < 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation. |
| وصف الملف: | text/html |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18662d1d9e97ab9418ff742c6de2bf32 http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....18662d1d9e97ab9418ff742c6de2bf32 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |