The effects of bariatric surgery on clinical profile, DNA methylation, and ageing in severely obese patients
| العنوان: | The effects of bariatric surgery on clinical profile, DNA methylation, and ageing in severely obese patients |
|---|---|
| المؤلفون: | Vincent W. Bloks, Ana B. Crujeiras, Eliza Fraszczyk, Mirjam Luijten, Jan Greve, Sander S. Rensen, Paul F.K. Wackers, Annemieke M.W. Spijkerman, Wim A. Buurman, Harold Snieder, Jana V. van Vliet-Ostaptchouk, Carla Barbosa Nonino, Carolina Ferreira Nicoletti, Bruce H. R. Wolffenbuttel |
| المساهمون: | Surgery, RS: NUTRIM - R2 - Liver and digestive health, Life Course Epidemiology (LCE), Lifestyle Medicine (LM), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Clinical Epigenetics Clinical epigenetics, 12(1):14. BioMed Central Ltd Clinical Epigenetics, 12(1):14. BMC |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Aging, Epigenetic clock, medicine.medical_treatment, Biological age, Type 2 diabetes, Epigenesis, Genetic, Morbid obesity, 0302 clinical medicine, Weight loss, Medicine, WIDE ASSOCIATION, ÍNDICE DE MASSA CORPORAL, Genetics (clinical), GENE-EXPRESSION, 2. Zero hunger, RISK, 0303 health sciences, OUTCOMES, DNA methylation, Middle Aged, 3. Good health, Obesity, Morbid, ADIPOSE-TISSUE, 030220 oncology & carcinogenesis, Female, Epigenetics, LIFE-STYLE, medicine.symptom, Adult, medicine.medical_specialty, MECHANISMS, 03 medical and health sciences, AGE, Genetics, Epigenetic Profile, MANAGEMENT, Humans, Obesity, Molecular Biology, 030304 developmental biology, EWAS, Bariatric surgery, business.industry, Insulin, Research, medicine.disease, Surgery, Ageing, CpG Islands, WEIGHT, business, Developmental Biology |
| الوصف: | Background Severe obesity is a growing, worldwide burden and conventional therapies including radical change of diet and/or increased physical activity have limited results. Bariatric surgery has been proposed as an alternative therapy showing promising results. It leads to substantial weight loss and improvement of comorbidities such as type 2 diabetes. Increased adiposity is associated with changes in epigenetic profile, including DNA methylation. We investigated the effect of bariatric surgery on clinical profile, DNA methylation, and biological age estimated using Horvath’s epigenetic clock. Results To determine the impact of bariatric surgery and subsequent weight loss on clinical traits, a cohort of 40 severely obese individuals (BMI = 30–73 kg/m2) was examined at the time of surgery and at three follow-up visits, i.e., 3, 6, and 12 months after surgery. The majority of the individuals were women (65%) and the mean age at surgery was 45.1 ± 8.1 years. We observed a significant decrease over time in BMI, fasting glucose, HbA1c, HOMA-IR, insulin, total cholesterol, triglycerides, LDL and free fatty acids levels, and a significant small increase in HDL levels (all p values < 0.05). Epigenome-wide association analysis revealed 4857 differentially methylated CpG sites 12 months after surgery (at Bonferroni-corrected p value < 1.09 × 10−7). Including BMI change in the model decreased the number of significantly differentially methylated CpG sites by 51%. Gene set enrichment analysis identified overrepresentation of multiple processes including regulation of transcription, RNA metabolic, and biosynthetic processes in the cell. Bariatric surgery in severely obese patients resulted in a decrease in both biological age and epigenetic age acceleration (EAA) (mean = − 0.92, p value = 0.039). Conclusions Our study shows that bariatric surgery leads to substantial BMI decrease and improvement of clinical outcomes observed 12 months after surgery. These changes explained part of the association between bariatric surgery and DNA methylation. We also observed a small, but significant improvement of biological age. These epigenetic changes may be modifiable by environmental lifestyle factors and could be used as potential biomarkers for obesity and in the future for obesity related comorbidities. |
| وصف الملف: | application/pdf |
| تدمد: | 1868-7075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18758fd625e0c55359ce138d0b7d7654 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....18758fd625e0c55359ce138d0b7d7654 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 18687075 |
|---|