Towards By-Product Utilisation of Pea Hulls: Isolation and Quantification of Galacturonic Acid
| العنوان: | Towards By-Product Utilisation of Pea Hulls: Isolation and Quantification of Galacturonic Acid |
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| المؤلفون: | Friederike, Gutöhrlein, Stephan, Drusch, Sebastian, Schalow |
| المصدر: | Foods, Vol 7, Iss 12, p 203 (2018) Foods Volume 7 Issue 12 |
| بيانات النشر: | Technische Universität Berlin, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 630 Landwirtschaft und verwandte Bereiche, Saeman hydrolysis, TFA, EDTA, food and beverages, polygalacturonase, pea hulls, lcsh:Chemical technology, Article, cellulose, 640 Hauswirtschaft und Familie, ddc:630, ddc:640, lcsh:TP1-1185, galacturonic acid |
| الوصف: | In order to evaluate by-products from food processing as alternative raw materials for pectin extraction, their amount of galacturonic acid (GalA) has to be analysed as a marker for pectin content. In the present study, significant differences in GalA release using different digestion methods are shown for pea hulls, as an example of by-products with a high content of cellulose. Complete digestion of the fibre matrix was assumed for Saeman hydrolysis as a reference protocol. Significantly lower GalA release was achieved by a treatment with trifluoracetic acid (TFA). An alternative treatment with ethylenediaminetetraacetic acid (EDTA) at pH 11 followed by an enzymatic digestion at pH 4.5 using a combination of polygalacturonase (Vegazyme M) and cellulase (Celluclast 1.5L) resulted in a similar release of GalA compared to Seaman hydolysis. Pea hull samples, analysed by this alternative protocol, showed on average a GalA content of 11.2%. Therefore, pea hulls may serve as new raw material for pectin extraction. |
| وصف الملف: | application/pdf |
| DOI: | 10.14279/depositonce-8211 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::188d85206199acec150f689b2f05cd55 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....188d85206199acec150f689b2f05cd55 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.14279/depositonce-8211 |
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