MiR130b-Regulation of PPARγ Coactivator- 1α Suppresses Fat Metabolism in Goat Mammary Epithelial Cells
| العنوان: | MiR130b-Regulation of PPARγ Coactivator- 1α Suppresses Fat Metabolism in Goat Mammary Epithelial Cells |
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| المؤلفون: | Dawei Yao, Jiangjiang Zhu, Zhi Chen, Jun Luo, Liuan Ma, Deming Gou, Kang Kang, Wenting Cao, Jun Li, HuiFei Xu, Yuting Sun, Hui Wang |
| المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 11, p e0142809 (2015) |
| بيانات النشر: | Public Library of Science, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cellular differentiation, lcsh:Medicine, Biology, chemistry.chemical_compound, Mammary Glands, Animal, Adipocyte, microRNA, Gene expression, Coactivator, Animals, Humans, lcsh:Science, Regulation of gene expression, Multidisciplinary, Adipogenesis, Goats, lcsh:R, Gene Expression Regulation, Developmental, Lipid metabolism, Cell Differentiation, Epithelial Cells, Lipid Metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Cell biology, MicroRNAs, Milk, Biochemistry, chemistry, lcsh:Q, Female, Research Article, Transcription Factors |
| الوصف: | Fat metabolism is a complicated process regulated by a series of factors. microRNAs (miRNAs) are a class of negative regulator of proteins and play crucial roles in many biological processes; including fat metabolism. Although there have been some researches indicating that miRNAs could influence the milk fat metabolism through targeting some factors, little is known about the effect of miRNAs on goat milk fat metabolism. Here we utilized an improved miRNA detection assay, S-Poly-(T), to profile the expression of miRNAs in the goat mammary gland in different periods, and found that miR-130b was abundantly and differentially expressed in goat mammary gland. Additionally, overexpressing miR-130b impaired adipogenesis while inhibiting miR-130b enhanced adipogenesis in goat mammary epithelial cells. Utilizing 3'-UTR assay and Western Blot analusis, the protein peroxisome proliferator-activated receptor coactivator-1α (PGC1α), a major regulator of fat metabolism, was demonstrated to be a potential target of miR-130b. Interestingly, miR-130b potently repressed PGC1α expression by targeting both the PGC1α mRNA coding and 3' untranslated regions. These findings have some insight of miR-130b in mediating adipocyte differentiation by repressing PGC1α expression and this contributes to further understanding about the functional significance of miRNAs in milk fat synthesis. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18c2ffe5e5d142fbfdf02a11b55ad7fe http://europepmc.org/articles/PMC4651502 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....18c2ffe5e5d142fbfdf02a11b55ad7fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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