Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium
| العنوان: | Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium |
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| المؤلفون: | Mónica B. Wachsman, Svetoslav Dimitrov Todorov, Elisabetta Tomé, Leon M. T. Dicks, Maria Teresa Destro, Bernadette Dora Gombossy de Melo Franco, Djamel Drider, X. Dousset, Manuella Vaz-Velho |
| المساهمون: | Franco, Bernadette Dora Gombossy de Melo, Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Universidade de São Paulo (USP), Department of Microbiology, Nippon Dental University, Universidad de Buenos Aires (UBA), Universidad Central de Venezuela (UCV), SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Instituto Politécnico, Universidade do Estado do Rio de Janeiro [Rio de Janeiro] (UERJ), CAPES, Brazilian Ministry of Education, Brasilia, Brazil |
| المصدر: | Food Microbiology 7 (27), 869-879. (2010) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Food Microbiology Food Microbiology, Elsevier, 2010, 27 (7), pp.869-879. ⟨10.1016/j.fm.2010.05.001⟩ |
| بيانات النشر: | Elsevier BV, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | pediocin-like-bacteriocin, enterococcus faecium, salmon, anti-Listeria activity, antiviral activity, Listeria, [SDV]Life Sciences [q-bio], Gram-positive bacteria, Colony Count, Microbial, Herpesvirus 1, Human, Microbial Sensitivity Tests, medicine.disease_cause, Antiviral Agents, Microbiology, food, Bacteriocins, Bacteriocin, Listeria monocytogenes, Antibiosis, medicine, Animals, Humans, biology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, food.food, Anti-Bacterial Agents, Molecular Weight, Smoked salmon, Seafood, Consumer Product Safety, SALMÃO, Food Microbiology, bacteria, Listeria ivanovii, Bacteria, Food Science, Enterococcus faecium |
| الوصف: | The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 degrees C and 37 degrees C, reaching a maximum production of 1.0 x 10(9) AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. |
| وصف الملف: | application/pdf |
| تدمد: | 0740-0020 1095-9998 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18d365fd00cee7f5cbb378fca8f49903 https://doi.org/10.1016/j.fm.2010.05.001 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....18d365fd00cee7f5cbb378fca8f49903 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07400020 10959998 |
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