Flavonoids from Machilus japonica stems and their inhibitory effects on LDL oxidation
| العنوان: | Flavonoids from Machilus japonica stems and their inhibitory effects on LDL oxidation |
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| المؤلفون: | Hee-Cheol Kang, Se-Jin Joo, Nam-In Baek, Hack-Soo Kim, Tae-Sook Jeong, Hee-Jung Park, Ji-Hae Park, Ji-Hyun Kang, Jin-Gyeong Cho, Sangyo Byun, Dae Young Lee |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 15, Iss 9, Pp 16418-16429 (2014) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(15): 9 |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | diphenyl picryl hydrazinyl, Antioxidant, DPPH, Machilus japonica, medicine.medical_treatment, Flavonoid, Ethyl acetate, Catalysis, Antioxidants, Inorganic Chemistry, lcsh:Chemistry, chemistry.chemical_compound, Lauraceae, medicine, Organic chemistry, Butylated hydroxytoluene, flavonoid, Lipoprotein oxidation, Afzelin, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, chemistry.chemical_classification, Flavonoids, Plant Stems, Communication, Organic Chemistry, General Medicine, Ascorbic acid, low-density lipoprotein-oxidation, NMR, Computer Science Applications, Lipoproteins, LDL, chemistry, lcsh:Biology (General), lcsh:QD1-999, Nuclear chemistry |
| الوصف: | Stems of Machilus japonica were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was successively extracted with ethyl acetate (EtOAc), normal butanol (n-BuOH), and water. Six flavonoids were isolated from the EtOAc fraction: (+)-taxifolin, afzelin, (-)-epicatechin, 5,3'-di-O-methyl-(-)-epicatechin, 5,7,3'-tri-O-methyl-(-)-epicatechin, and 5,7-di-O-methyl-3', 4'-methylenedioxyflavan-3-ol. The chemical structures were identified using spectroscopic data including NMR, mass spectrometry and infrared spectroscopy. This is the first report of isolation of these six compounds from M. japonica. The compounds were evaluated for their diphenyl picryl hydrazinyl scavenging activity and inhibitory effects on low-density lipoprotein oxidation. Compounds 1 and 3-6 exhibited DPPH antioxidant activity equivalent with that of ascorbic acid, with half maximal inhibitory concentration (IC50) values of 0.16, 0.21, 0.17, 0.15 and 0.07 mM, respectively. The activity of compound 1 was similar to the positive control butylated hydroxytoluene, which had an IC50 value of 1.9 mu M, while compounds 3 and 5 showed little activity. Compounds 1, 3, and 5 exhibited LDL antioxidant activity with IC50 values of 2.8, 7.1, and 4.6 mu M, respectively. |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::196c099bc936ed428c8be16573f9c6e4 https://pubmed.ncbi.nlm.nih.gov/25229822 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....196c099bc936ed428c8be16573f9c6e4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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