Lumpfish ( Cyclopterus lumpus, Linnaeus) is susceptible to viral nervous necrosis: Result of an experimental infection with different genotypes of Betanodavirus
| العنوان: | Lumpfish ( Cyclopterus lumpus, Linnaeus) is susceptible to viral nervous necrosis: Result of an experimental infection with different genotypes of Betanodavirus |
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| المؤلفون: | Alessandra Buratin, Maria De Salvador, Hamish D. Rodger, Felix Scholz, Tobia Pretto, Argelia Cuenca, Marica Toson, Niccolò Vendramin, Anna Toffan |
| المصدر: | Toffan, A, De Salvador, M, Scholz, F, Pretto, T, Buratin, A, Rodger, H D, Toson, M, Cuenca, A & Vendramin, N 2019, ' Lumpfish ( Cyclopterus lumpus, Linnaeus) is susceptible to viral nervous necrosis: Result of an experimental infection with different genotypes of Betanodavirus ', Journal of Fish Diseases, vol. 42, no. 12, pp. 1667-1676 . https://doi.org/10.1111/jfd.13088 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Necrosis, Genotype, Veterinary (miscellaneous), Cyclopterus lumpus, Betanodavirus, Aquatic Science, Cleaner fish, Virus, Fish Diseases, 03 medical and health sciences, RNA Virus Infections, SDG 3 - Good Health and Well-being, medicine, Animals, Nodaviridae, 14. Life underwater, biology, Norway, 04 agricultural and veterinary sciences, biology.organism_classification, Virology, Perciformes, 3. Good health, 030104 developmental biology, Lumpfish, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Nervous necrosis virus, Histopathology, Disease Susceptibility, medicine.symptom, Viral load, Viral encephalopathy and retinopathy |
| الوصف: | In recent years, the use of cleaner fish for biological control of sea lice has increased considerably. Along with this, a number of infectious diseases have emerged. The aim of this study was to investigate the susceptibility of lumpfish (Cyclopterus lumpus) to Betanodavirus since it was detected in asymptomatic wild wrasses in Norway and Sweden. Three betanodaviruses were used to challenge lumpfish: one RGNNV genotype and two BFNNV genotypes. Fish were injected and monitored for 4 weeks. Brain samples from clinically affected specimens, from weekly randomly selected fish and survivors were subjected to molecular testing, viral isolation, histopathology and immunohistochemistry. Reduced survival was observed but was attributed to tail-biting behaviour, since no nervous signs were observed throughout the study. Betanodavirus RNA was detected in all samples, additionally suggesting an active replication of the virus in the brain. Viral isolation confirmed molecular biology results and revealed a high viral titre in BFNNV-infected groups associated with typical lesions in brains and eyes of survivor fish. We concluded that lumpfish are susceptible to Betanodavirus, as proven by the high viral titre and brain lesions detected, but further studies are necessary to understand if Betanodavirus can cause clinical disease in this species. |
| وصف الملف: | application/pdf |
| تدمد: | 1365-2761 0140-7775 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::197f313b54a34b5c772da9030472c308 https://doi.org/10.1111/jfd.13088 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....197f313b54a34b5c772da9030472c308 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652761 01407775 |
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