Insecta is the most diverse and largest class of animals on Earth, appearing together with the emergence of the first terrestrial ecosystem. Owing to this great diversity and long-term coexistence, an amazing variety of symbiotic microorganisms have adapted specifically to insects as hosts. Insect symbionts not only participate in many relationships with the hosts but also represent a novel resource for biotechnological applications. The exploitation of mutualistic symbiosis represents a promising area to search for bioactive compounds and new enzymes for potential clinical, industrial or environmental applications. Moreover, the manipulation of parasitic symbiosis has particular potential to solve practical problems for the control of agricultural pests and disease vectors. Although the study of microbial symbionts has been impaired by the unculturability of most symbionts, the rapidly growing catalogue of microbial genomes and the application of modern genetic techniques provide an alternative approach to using these microbes. This minireview presents examples of microbial symbionts isolated from insects for emerging biotechnological use and illuminates new ways for discovering microorganisms of applied value from a particularly promising source.