Cadmium-induced Cytosolic Ca2+ Elevation and Subsequent Apoptosis in Renal Tubular Cells
| العنوان: | Cadmium-induced Cytosolic Ca2+ Elevation and Subsequent Apoptosis in Renal Tubular Cells |
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| المؤلفون: | Chorng-Chih Huang, Mei-Yin Yeh, Chung-Ren Jan, Jeng-Hsien Yeh, Jenn-Kuen Lee, Jyh-Seng Wang |
| المصدر: | Basic & Clinical Pharmacology & Toxicology. 104:345-351 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | medicine.medical_specialty, Thapsigargin, Cell Survival, chemistry.chemical_element, Apoptosis, Phospholipase, Calcium, Toxicology, chemistry.chemical_compound, Cytosol, Dogs, BAPTA, Internal medicine, Cadmium Compounds, medicine, Extracellular, Animals, Magnesium, Channel blocker, Propidium iodide, Cells, Cultured, Pharmacology, Endoplasmic reticulum, General Medicine, Calcium Channel Blockers, Molecular biology, Kidney Tubules, Endocrinology, chemistry, Type C Phospholipases, Environmental Pollutants |
| الوصف: | Cadmium (Cd 2+ ) is an industrial and environmental metal. The effect of Cd 2+ on intracellular free-Ca 2+ levels ([Ca 2+ ] i ) and viability in Madin Darby canine kidney cells was explored. Cd 2+ increased [Ca 2+ ] i in a concentration-dependent manner with an EC 50 of 85 μM. Cd 2+ -induced Mn 2+ entry demonstrated Ca 2+ influx. Removal of extracellular Ca 2+ decreased the [Ca 2+ ] i signal by 60%. The [Ca 2+ ] i signal was inhibited by La 3+ but not by L-type Ca 2+ channel blockers. In Ca 2+ -free medium, Cd 2+ -induced [Ca 2+ ] i signal was abolished by pre-treatment with 1 μM thapsigargin (an endoplasmic reticulum Ca 2+ pump inhibitor) and 2 μM carbonylcyanide m-chlorophenylhydrazone (CCCP; a mitochondrial uncoupler). Cd 2+ -induced Ca 2+ release was not altered by inhibition of phospholipase C. At concentrations between 10 and 100 μM, Cd 2+ killed cells in a concentration-dependent manner. The cytotoxic effect of 100 μM Cd 2+ was reversed by pre-chelating cytosolic Ca 2+ with BAPTA. Cd 2+ -induced apoptosis was demonstrated by propidium iodide. Collectively, this study shows that Cd 2+ induced a [Ca 2+ ], increase in Madin Darby canine kidney cells via evoking Ca 2+ entry through non-selective Ca 2+ channels, and releasing stored Ca 2+ from endoplasmic reticulum and mitochondria in a phospholipase C-independent manner. |
| تدمد: | 1742-7843 1742-7835 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19a1bbefb408b31caac82ff62e8a62bf https://doi.org/10.1111/j.1742-7843.2009.00391.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....19a1bbefb408b31caac82ff62e8a62bf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17427843 17427835 |
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