Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide
| العنوان: | Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide |
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| المؤلفون: | Patrick Thomas, Carina Walpole, Angela O'Keeffe, Eliza Whiteside, Penny L. Jeffery, Lisa K. Chopin, Pei-Yi Yap, Michelle Maugham, Inge Seim, Adrian C. Herington, Jenny N. Fung, John Lai |
| المصدر: | Endocrine |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Comparative endocrinology, Evolution, Endocrinology, Diabetes and Metabolism, media_common.quotation_subject, Peptide hormone, Biology, Mice, 03 medical and health sciences, Exon, Endocrinology, Species Specificity, Cell Movement, Animals, Humans, Protein Isoforms, splice, Amino Acid Sequence, Gene, Cells, Cultured, Conserved Sequence, Cell Proliferation, media_common, Genetics, Appetite Regulation, digestive, oral, and skin physiology, Alternative splicing, Appetite, Ghrelin, 3. Good health, Mice, Inbred C57BL, Alternative Splicing, 030104 developmental biology, Original Article, hormones, hormone substitutes, and hormone antagonists, Function (biology) |
| الوصف: | The peptide hormone ghrelin is a potent orexigen produced predominantly in the stomach. It has a number of other biological actions, including roles in appetite stimulation, energy balance, the stimulation of growth hormone release and the regulation of cell proliferation. Recently, several ghrelin gene splice variants have been described. Here, we attempted to identify conserved alternative splicing of the ghrelin gene by cross-species sequence comparisons. We identified a novel human exon 2-deleted variant and provide preliminary evidence that this splice variant and in1-ghrelin encode a C-terminally truncated form of the ghrelin peptide, termed minighrelin. These variants are expressed in humans and mice, demonstrating conservation of alternative splicing spanning 90 million years. Minighrelin appears to have similar actions to full-length ghrelin, as treatment with exogenous minighrelin peptide stimulates appetite and feeding in mice. Forced expression of the exon 2-deleted preproghrelin variant mirrors the effect of the canonical preproghrelin, stimulating cell proliferation and migration in the PC3 prostate cancer cell line. This is the first study to characterise an exon 2-deleted preproghrelin variant and to demonstrate sequence conservation of ghrelin gene-derived splice variants that encode a truncated ghrelin peptide. This adds further impetus for studies into the alternative splicing of the ghrelin gene and the function of novel ghrelin peptides in vertebrates. Electronic supplementary material The online version of this article (doi:10.1007/s12020-015-0848-7) contains supplementary material, which is available to authorized users. |
| تدمد: | 1559-0100 1355-008X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19d297402f592b8b76fc90e953f2dc10 https://doi.org/10.1007/s12020-015-0848-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....19d297402f592b8b76fc90e953f2dc10 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15590100 1355008X |
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