Optimized two‐step electroporation process to achieve efficient nonviral‐mediated gene insertion into primary T cells
| العنوان: | Optimized two‐step electroporation process to achieve efficient nonviral‐mediated gene insertion into primary T cells |
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| المؤلفون: | Laurent Poirot, Alexandre Juillerat, Diane Tkach, Ming Yang, Philippe Duchateau, Selena Kazancioglu, Aymeric Duclert, Alex Boyne |
| المصدر: | FEBS Open Bio, Vol 12, Iss 1, Pp 38-50 (2022) FEBS Open Bio |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Transcription activator-like effector nuclease, Chemistry, gene editing, QH301-705.5, T-Lymphocytes, Electroporation, Transfection, chimeric antigen receptors, nonviral vectorization, General Biochemistry, Genetics and Molecular Biology, Chimeric antigen receptor, Cell biology, Viral vector, Mutagenesis, Insertional, Genome editing, TALEN, Naked DNA, Insertion, Biology (General), Insight, double‐stranded break |
| الوصف: | Gene editing enables scientists to make precise changes to the genome of an organism using the cell’s own ability to repair damaged DNA using a supplied DNA template. In recent years, gene editing has been applied clinically in the treatment of diseases such as cancer. Gene editing has been used in a type of immunotherapy, known as chimeric antigen receptor‐expressing T cell (CAR‐T) therapy, to restore the body’s ability to find and kill specific cancer cells. For this therapy, viruses are often used to supply the cell with the DNA template used for creating the edit in the target DNA. However, the use of viruses in this context is laborious and costly. Developing non‐viral methods for delivery of DNA templates for gene editing would circumvent these problems, but current methods can have toxic effects on cells and result in low editing efficiency. In a new article published in this issue, Yang et al. describe a novel method for viral‐independent delivery of naked DNA and its incorporation into the genome for engineering cells for CAR‐T therapy. Gene editing can be used to restore the body’s ability to kill specific cancer cells. Viruses are often used to supply the cell with the DNA template used for creating the edit in the target DNA, but this is laborious and costly. In this issue, Yang et al. describe a novel method for viral‐independent delivery of naked DNA for engineering cells for CAR‐T therapy. |
| اللغة: | English |
| تدمد: | 2211-5463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19d48658e4280aca4614dcbe916de048 https://doaj.org/article/b489919778d241b5b206cd7cf268dbe0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....19d48658e4280aca4614dcbe916de048 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22115463 |
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