Electrospray ionization mass spectrometry of synthetic oligonucleotides using 2-propanol and spermidine
| العنوان: | Electrospray ionization mass spectrometry of synthetic oligonucleotides using 2-propanol and spermidine |
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| المؤلفون: | Piergiorgio Pietta, Enrico Rosti, Gianluca De Bellis, Pierluigi Mauri, Cristina Battaglia, Giuliana Salani |
| المصدر: | Rapid Communications in Mass Spectrometry. 14:243-249 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Chromatography, Protein mass spectrometry, Chemistry, Oligonucleotide, Electrospray ionization, Organic Chemistry, Extractive electrospray ionization, Capillary electrophoresis–mass spectrometry, Sample preparation in mass spectrometry, Analytical Chemistry, Adduct, DNA, performance, Settore BIO/10 - Biochimica, Direct electron ionization liquid chromatography–mass spectrometry interface, Spectroscopy |
| الوصف: | Oligonucleotides have become widely used tools in molecular biology and molecular diagnostics. Their parallel synthesis in large numbers and the increasing interest in microarray technology has raised the requirement for fast and informative analytical tools for their quality control. A direct injection electrospray ionization mass spectrometry (ESI-MS) technique based on the use of aqueous 2-propanol as running eluent, and spermidine (or triethylamine) as DNA modifiers, has been applied to analyze a large set of samples (about 200 synthetic oligonucleotides) ranging from 5 to 15 kDa (17-51mers) with good results in terms of sensitivity, suppression of sodium adduct formation, and speed of analysis. Copyright 2000 John Wiley & Sons, Ltd. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19db2ff6b38ced9b2a735a170d36170b https://doi.org/10.1002/(sici)1097-0231(20000229)14:4<243::aid-rcm870>3.0.co;2-f |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....19db2ff6b38ced9b2a735a170d36170b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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