Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer
| العنوان: | Clinicopathological utility of miR-203a-3p in diagnosing colorectal cancer |
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| المؤلفون: | Mahdi Qasemi Rad, Vahid Pouresmaeil, Fatemeh Hosseini Mojahed, Amir Amirabadi, Amir Hossein Aalami |
| المصدر: | Molecular Biology Reports. 49:6975-6985 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Gene Expression Regulation, Neoplastic, MicroRNAs, Case-Control Studies, Lymphatic Metastasis, Biomarkers, Tumor, Genetics, Humans, General Medicine, Neoplasm Recurrence, Local, Colorectal Neoplasms, Molecular Biology |
| الوصف: | Biomarkers, such as microRNAs, are helpful in diagnosing colorectal cancer, regulating disease progression, predicting disease recurrence, and determining therapy success. This research aimed to look at the clinicopathological characteristics of serum miRNA-203a-3p expression in colorectal cancer patients.This case-control study was conducted on 43 patients with colorectal cancer and 43 healthy individuals. After RNA extraction, cDNA was synthesized. The expression of miR-203a-3p was measured using RT-qPCR. Demographic and histochemical data were extracted from patient documents. SPSS and GraphPad Prism software were used to analyze the data. The expression of miR-203a-3p in CRC patients was 2.39 times lower than in the control group (p 0.0001). The miR-203a-3p expression was significantly lower in the CRC tumor stages, tumor grades, and lymph node metastasis compared to the control group (p 0.0001 each). The ROC curves showed that the AUC was 0.73, and the best cut-point based on the Youden index was 0.3954, 0.7105, 0.5087, and 0.4868 for detecting colorectal cancer (p = 0.0002), tumor grade (p = 0.006), tumor stage (p = 0.001), and lymph node metastasis (p = 0.0011) compared to the control group, respectively. The binary logistic regression analysis was performed on the correlation between BMI, smoking, and cancer inheritance with miR-203a-3p in cancer and control groups.This study's findings revealed that serum miR-203a-3p is a fair non-invasive molecular biomarker for diagnosing and progressing tumor grade, tumor stage, and lymph node metastasis in colorectal cancer. However, further research with higher statistical numbers is needed to strengthen the correlation and be used for diagnostic applications. |
| تدمد: | 1573-4978 0301-4851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19eac6fdb287efabeb1e08767b753daf https://doi.org/10.1007/s11033-022-07465-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....19eac6fdb287efabeb1e08767b753daf |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734978 03014851 |
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