Facile One Pot Greener Synthesis of Sophorolipid Capped Gold Nanoparticles and its Antimicrobial Activity having Special Efficacy Against Gram Negative Vibrio cholerae
| العنوان: | Facile One Pot Greener Synthesis of Sophorolipid Capped Gold Nanoparticles and its Antimicrobial Activity having Special Efficacy Against Gram Negative Vibrio cholerae |
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| المؤلفون: | Saumya Ray Chaudhuri, Mani Shankar Bhattacharyya, Sristy Shikha |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.drug_class, Antibiotics, lcsh:Medicine, Metal Nanoparticles, Oleic Acids, 02 engineering and technology, Cell Growth Processes, medicine.disease_cause, Article, 03 medical and health sciences, Anti-Infective Agents, Cholera, medicine, lcsh:Science, Vibrio cholerae, Multidisciplinary, Chemistry, Sophorolipid, lcsh:R, Cell Membrane, Biofilm, Green Chemistry Technology, 021001 nanoscience & nanotechnology, Antimicrobial, 030104 developmental biology, Biochemistry, Colloidal gold, Staphylococcus aureus, Biofilms, Nanomedicine, Nanoparticles, lcsh:Q, Gold, 0210 nano-technology |
| الوصف: | Microbes develop several strategies to survive in the adverse condition such as biofilm formation, attaining non-dividing state, altering drug target or drug, thereby increases the burden of drug dosage. To combat these issues, nanoparticles have shown an alternative approach for new treatment strategy but synthesis via chemical synthetic route limits their application in biomedical field. Here, green method for the synthesis of gold nanoparticles using sophorolipid (SL) is discussed that is characterized by various techniques. Initially, the antimicrobial activity was checked against metabolically active state of microbes; Gram-positive Staphylococcus aureus and Gram-negative Vibrio cholerae using XTT assay and growth kinetics assay. Results suggested higher efficacy of nanoparticles for Gram-negative, therefore further analyzed against Escherichia coli that confirmed its potency for the same. AuNPs-SL also signifies its efficiency at least metabolically active state; non dividing cells and biofilm of these microbes. Induced morphological changes were studied by SEM that revealed AuNPs-SL led to disruption of cell membrane and leakage of intracellular fluid to the surroundings. Inhibition of respiratory enzymes activity also plays a crucial role in bactericidal action as indicated by LDH assay. Synergy of AuNPs-SL with different antibiotics was also analyzed using checkerboard assay. These results suggested the possible use of AuNPs-SL as an antimicrobial therapy in the field of nanomedicine. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ad7e35362d1e83cf46a4caad7b32b85 https://pubmed.ncbi.nlm.nih.gov/31996706 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1ad7e35362d1e83cf46a4caad7b32b85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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