Mechanisms of Danggui Buxue Tang on Hematopoiesis via Multiple Targets and Multiple Components: Metabonomics Combined with Database Mining Technology
| العنوان: | Mechanisms of Danggui Buxue Tang on Hematopoiesis via Multiple Targets and Multiple Components: Metabonomics Combined with Database Mining Technology |
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| المؤلفون: | Defu, Tie, Zhaohui, Fan, Dan, Chen, Xiao, Chen, Qizhu, Chen, Jun, Chen, Huaben, Bo |
| المصدر: | The American Journal of Chinese Medicine. 50:1155-1171 |
| بيانات النشر: | World Scientific Pub Co Pte Ltd, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mice, Complementary and alternative medicine, Databases, Pharmaceutical, Animals, Cytochrome P-450 CYP3A, Data Mining, Metabolomics, General Medicine, Anti-Bacterial Agents, Drugs, Chinese Herbal, Hematopoiesis |
| الوصف: | This study aimed to explore the mechanism of action of Danggui Buxue Tang (DBT) with its multiple components and targets in the synergistic regulation of hematopoiesis. Mouse models of hematopoiesis were established using antibiotics. Metabolomics was used to detect body metabolites and enriched pathways. The active ingredients, targets, and pathways of DBT were analyzed using system pharmacology. The results of metabolomics and system pharmacology were integrated to identify the key pathways and targets. A total of 515 metabolites were identified using metabolomics. After the action of antibiotics, 49 metabolites were markedly changed: 23 were increased, 26 were decreased, and 11 were significantly reversed after DBT administration. Pathway enrichment analysis showed that these 11 metabolites were related to bile secretion, cofactor biosynthesis, and fatty acid biosynthesis. The results of the pharmacological analysis showed that 616 targets were related to DBT-induced anemia, which were mainly enriched in biological processes, such as bile secretion, biosynthesis of cofactors, and cholesterol metabolism. Combined with the results of metabolomics and system pharmacology, we found that bile acid metabolism and biotin synthesis were the key pathways for DBT. Forty-two targets of DBT were related to these two metabolic pathways. PPI analysis revealed that the top 10 targets were CYP3A4, ABCG2, and UGT1A8. Twenty-one components interacted with these 10 targets. In one case, a target corresponds to multiple components, and a component corresponds to multiple targets. DBT acts on multiple targets of ABCG2, UGT1A8, and CYP3A4 through multiple components, affecting the biosynthesis of cofactors and bile secretion pathways to regulate hematopoiesis. |
| تدمد: | 1793-6853 0192-415X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1addde7aa596cd266a5a80096784c98e https://doi.org/10.1142/s0192415x22500471 |
| رقم الأكسشن: | edsair.doi.dedup.....1addde7aa596cd266a5a80096784c98e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17936853 0192415X |
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