Synovial cell cross-talk with cartilage plays a major role in the pathogenesis of osteoarthritis
| العنوان: | Synovial cell cross-talk with cartilage plays a major role in the pathogenesis of osteoarthritis |
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| المؤلفون: | Virginia B. Kraus, David E. Attarian, Jason Gibson, Simon G. Gregory, Ching-Heng Chou, C. Haraden, Remi-Martin Laberge, Christopher B. Yohn, Vaibhav Jain |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cartilage, Articular, Male, Cell type, Molecular biology, medicine.medical_treatment, lcsh:Medicine, Arthritis, Osteoarthritis, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Rheumatic diseases, medicine, Synovial fluid, Humans, RNA-Seq, lcsh:Science, Cells, Cultured, Aged, 030203 arthritis & rheumatology, Multidisciplinary, Cartilage, lcsh:R, RNA sequencing, medicine.disease, Synoviocytes, Cell biology, TLR2, 030104 developmental biology, Cytokine, medicine.anatomical_structure, Synovial Cell, Case-Control Studies, lcsh:Q, Female, Biomarkers |
| الوصف: | We elucidated the molecular cross-talk between cartilage and synovium in osteoarthritis, the most widespread arthritis in the world, using the powerful tool of single-cell RNA-sequencing. Multiple cell types were identified based on profiling of 10,640 synoviocytes and 26,192 chondrocytes: 12 distinct synovial cell types and 7 distinct articular chondrocyte phenotypes from matched tissues. Intact cartilage was enriched for homeostatic and hypertrophic chondrocytes, while damaged cartilage was enriched for prefibro- and fibro-, regulatory, reparative and prehypertrophic chondrocytes. A total of 61 cytokines and growth factors were predicted to regulate the 7 chondrocyte cell phenotypes. Based on production by > 1% of cells, 55% of the cytokines were produced by synovial cells (39% exclusive to synoviocytes and not expressed by chondrocytes) and their presence in osteoarthritic synovial fluid confirmed. The synoviocytes producing IL-1beta (a classic pathogenic cytokine in osteoarthritis), mainly inflammatory macrophages and dendritic cells, were characterized by co-expression of surface proteins corresponding to HLA-DQA1, HLA-DQA2, OLR1 or TLR2. Strategies to deplete these pathogenic intra-articular cell subpopulations could be a therapeutic option for human osteoarthritis. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c3f1bcb5ed637df321ce8fed80827e5 https://pubmed.ncbi.nlm.nih.gov/32616761 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1c3f1bcb5ed637df321ce8fed80827e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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