Type IIb Heat Labile Enterotoxin B Subunit as a Mucosal Adjuvant to Enhance Protective Immunity against H5N1 Avian Influenza Viruses
| العنوان: | Type IIb Heat Labile Enterotoxin B Subunit as a Mucosal Adjuvant to Enhance Protective Immunity against H5N1 Avian Influenza Viruses |
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| المؤلفون: | Chun-Yi Lu, Bor-Luen Chiang, Ming-Hsi Huang, Chung-Chu Chen, Li-Min Huang, Ting-Hsuan Chen, Suh-Chin Wu, Chung-Hsiung Huang, Jia-Tsrong Jan, Neos Tang, Shih-Che Sue |
| المصدر: | Vaccines Vaccines, Vol 8, Iss 710, p 710 (2020) Volume 8 Issue 4 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, H5N1 vaccine, medicine.drug_class, medicine.medical_treatment, Immunology, lcsh:Medicine, Heat-labile enterotoxin, Monoclonal antibody, medicine.disease_cause, Article, Virus, 03 medical and health sciences, 0302 clinical medicine, Drug Discovery, medicine, Pharmacology (medical), Neutralizing antibody, Pharmacology, biology, lcsh:R, mucosal adjuvant, intranasal immunization, Virology, Influenza A virus subtype H5N1, Vaccination, 030104 developmental biology, Infectious Diseases, LTIIb-B5, biology.protein, Adjuvant, 030215 immunology |
| الوصف: | Human infections with highly pathogenic avian influenza H5N1 viruses persist as a major global health concern. Vaccination remains the primary protective strategy against H5N1 and other novel avian influenza virus infections. We investigated the use of E. coli type IIb heat labile enterotoxin B subunit (LTIIb-B5) as a mucosal adjuvant for intranasal immunizations with recombinant HA proteins against H5N1 avian influenza viruses. Use of LTIIb-B5 adjuvant elicited more potent IgG, IgA, and neutralizing antibody titers in both sera and bronchoalveolar lavage fluids, thus increasing protection against lethal virus challenges. LTIIb-B5 mucosal adjuvanticity was found to trigger stronger Th17 cellular response in spleen lymphocytes and cervical lymph nodes. Studies of anti-IL-17A monoclonal antibody depletion and IL-17A knockout mice also suggest the contribution from Th17 cellular response to anti-H5N1 protective immunity. Our results indicate a link between improved protection against H5N1 live virus challenges and increased Th17 response due to the use of LTIIb-B5 mucosal adjuvant with HA subunit proteins. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-393X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d12c159bb2a589ca8bc4c3ed2ccf649 https://doi.org/10.3390/vaccines8040710 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1d12c159bb2a589ca8bc4c3ed2ccf649 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2076393X |
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