Sex identification by polymerase chain reaction using X-Y homologous primer

التفاصيل البيبلوغرافية
العنوان: Sex identification by polymerase chain reaction using X-Y homologous primer
المؤلفون: Yutaka Nakahori, Yasuo Nakagome, Koichi Hamano, Makoto Iwaya
المصدر: American journal of medical genetics. 39(4)
سنة النشر: 1991
مصطلحات موضوعية: Male, Sex Determination Analysis, X Chromosome, Base Sequence, Touchdown polymerase chain reaction, Inverse polymerase chain reaction, Molecular Sequence Data, DNA, Single-Stranded, Biology, Y chromosome, Molecular biology, Polymerase Chain Reaction, law.invention, law, Sequence Homology, Nucleic Acid, Y Chromosome, Multiplex polymerase chain reaction, Humans, Female, Primer (molecular biology), Nested polymerase chain reaction, Genetics (clinical), Polymerase chain reaction
الوصف: A method of sex identification using the polymerase chain reaction technique is described. Using a pair of nucleotide primers from an X-Y homologous region, both the X and the Y sequences can be amplified simultaneously, and more importantly, they result in fragments of different lengths. The success of the procedure is therefore monitored by the presence of a X-specific band while sex is identified by the presence or absence of a Y-specific band.
تدمد: 0148-7299
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d19061cfbe5bfd68bb1fc37eb56a574
https://pubmed.ncbi.nlm.nih.gov/1877627
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....1d19061cfbe5bfd68bb1fc37eb56a574
قاعدة البيانات: OpenAIRE