Neuronal pentraxin II (NPTX2) Is Frequently Down-Regulated by Promoter Hypermethylation in Pancreatic Cancers
| العنوان: | Neuronal pentraxin II (NPTX2) Is Frequently Down-Regulated by Promoter Hypermethylation in Pancreatic Cancers |
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| المؤلفون: | Ling Zhang, Yan-fang Gong, Jun Gao, Zhao-Shen Li |
| المصدر: | Digestive Diseases and Sciences. 57:2608-2614 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Physiology, Down-Regulation, DNA Methyltransferase Inhibitor, Nerve Tissue Proteins, Adenocarcinoma, Biology, Polymerase Chain Reaction, Cell Line, Tumor, Pancreatic cancer, Gene expression, Biomarkers, Tumor, medicine, Humans, Gene silencing, Gene Silencing, RNA, Messenger, Promoter Regions, Genetic, Gastroenterology, Promoter, Methyltransferases, Methylation, DNA Methylation, medicine.disease, Molecular biology, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms, C-Reactive Protein, DNA methylation, Cancer research, Histone deacetylase |
| الوصف: | Gene silencing via promoter hypermethylation plays a crucial role in the pathogenesis of cancers. Neuronal pentraxin II (NPTX2) has been observed to be hypermethylated in pancreatic cancers. Methylation of NPTX2 might provide a novel diagnostic marker for pancreatic cancers. The objective of this study is to investigate the abnormal patterns of DNA methylation of NPTX2 in pancreatic cancers, and its role in the transcriptional silencing of NPTX2. NPTX2 expression was detected by reverse-transcription polymerase chain reaction (RT-PCR), and the methylation status of NPTX2 was assessed by methylation-specific polymerase chain reaction (MSP). Immunohistochemistry was used to examine the NPTX2 protein expression. The pancreatic cancer cell lines were treated with the DNA methyltransferase inhibitor, histone deacetylase inhibitors, either alone or in combination. The MSP analysis revealed that the promoter region of NPTX2 gene was largely unmethylated in normal pancreatic tissues, while NPTX2 was frequently hypermethylated in pancreatic cancer cells and in primary pancreatic carcinomas. Quantitative RT-PCR revealed that the mean mRNA expression level of NPTX2 in the pancreatic cancer tissues was significantly lower than that in the paired adjacent normal tissues (0.96 ± 0.91 vs. 2.78 ± 1.42, P |
| تدمد: | 1573-2568 0163-2116 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d31722d57c6f33c5ffaca78bcae1739 https://doi.org/10.1007/s10620-012-2202-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....1d31722d57c6f33c5ffaca78bcae1739 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15732568 01632116 |
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