Systematic Investigation of Metabolic Oligosaccharide Engineering Efficiency in Intestinal Cells Using a Dibenzocyclooctyne‐Monosaccharide Conjugate
| العنوان: | Systematic Investigation of Metabolic Oligosaccharide Engineering Efficiency in Intestinal Cells Using a Dibenzocyclooctyne‐Monosaccharide Conjugate |
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| المؤلفون: | Yuen Yi Lam, Angel Tan, Cameron J. Nowell, Kristian Kempe, Ben J. Boyd |
| المصدر: | Lam, Y Y, Tan, A, Nowell, C J, Kempe, K & Boyd, B J 2023, ' Systematic Investigation of Metabolic Oligosaccharide Engineering Efficiency in Intestinal Cells Using a Dibenzocyclooctyne-Monosaccharide Conjugate ', ChemBioChem, vol. 24, no. 12, e202300144 . https://doi.org/10.1002/cbic.202300144 |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Organic Chemistry, Molecular Medicine, bioorthogonal targeting, intestinal cells, dibenzocyclooctyne, metabolic glycan labelling, metabolic oligosaccharide engineering, Molecular Biology, Biochemistry |
| الوصف: | Metabolic oligosaccharide engineering (MOE) of cells with synthetic monosaccharides can introduce functionality to the glycans of cell membranes. Unnatural sugars (e. g., peracetylated mannose-azide) can be expressed on the cell surface with the azide group in place. After MOE, the azide group can participate in a copper-free click reaction with an alkyne (e. g., dibenzocyclooctyne, DBCO) probe. This allows the metabolic fate of monosaccharides in cells to be understood. However, in a drug delivery context it is desirable to have azide groups on the probe (e. g. a drug delivery particle) and the alkyne (e. g. DBCO) on the cell surface. Consequently, the labelling efficiency of intestinal cell lines (Caco-2 and HT29-MTX-E12) treated with N-dibenzocyclooctyne-tetra-acetylmannosamine, and the concentration- and time-dependent labelling were determined. Furthermore, the labelling of mucus in HT29-MTX-E12 cells with DBCO was shown. This study highlights the potential for using MOE to target azide-functionalised probes to intestinal tissues for drug delivery applications. |
| وصف الملف: | application/pdf |
| تدمد: | 1439-7633 1439-4227 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d574fc1708c080f088313ef2219cfc2 https://doi.org/10.1002/cbic.202300144 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1d574fc1708c080f088313ef2219cfc2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14397633 14394227 |
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