Bimodal magnetic resonance and optical imaging of extracellular matrix remodelling by orthotopic ovarian tumours
| العنوان: | Bimodal magnetic resonance and optical imaging of extracellular matrix remodelling by orthotopic ovarian tumours |
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| المؤلفون: | Liat Fellus-Alyagor, Dafna Ketter, Filip Bochner, Inbal E. Biton, Michal Neeman, Ofra Golani |
| المصدر: | British Journal of Cancer British Journal of Cancer, 123 (2) |
| بيانات النشر: | Nature Publishing Group UK, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, Stromal cell, Ovary, Article, Fluorescence imaging, 030218 nuclear medicine & medical imaging, Extracellular matrix, 03 medical and health sciences, Mice, 0302 clinical medicine, Stroma, Fibrosis, Cell Line, Tumor, Magnetic resonance imaging, medicine, Tumor Microenvironment, Animals, Humans, Ovarian Neoplasms, medicine.diagnostic_test, Chemistry, Optical Imaging, medicine.disease, Magnetic Resonance Imaging, Extracellular Matrix, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Heterografts, Female, Collagen, Ovarian cancer, Intravital microscopy |
| الوصف: | Background The extracellular matrix modulates the development of ovarian tumours. Currently, evaluation of the extracellular matrix in the ovary is limited to histological methods. Both magnetic resonance imaging (MRI) and two-photon microscopy (2PM) enable dynamic visualisation and quantification of fibrosis by endogenous contrast mechanisms: magnetisation transfer (MT) MRI and second-harmonic generation (SHG) 2PM, respectively. Methods Here, we applied the MT-MRI protocol for longitudinal imaging of the stroma in orthotopic human ovarian cancer ES-2 xenograft model in CD1 athymic nude mice, and for orthotopically implanted ovarian PDX using a MR-compatible imaging window chamber implanted into NSG mice. Results We observed differences between ECM deposition in ovarian and skin lesions, and heterogeneous collagen distribution in ES-2 lesions. An MR-compatible imaging window chamber enabled visual matching between T2 MRI maps of orthotopically implanted PDX grafts and anatomical images of their microenvironment acquired with a stereomicroscope and SHG–2PM intravital microscopy of the collagen. Bimodal MRI/2PM imaging allowed us to quantify the fibrosis within the same compartments, and demonstrated the consistent results across the modalities. Conclusions This work demonstrates a novel approach for measuring the stromal biomarkers in orthotopic ovarian tumours in mice, on both macroscopic and microscopic levels. British Journal of Cancer, 123 (2) ISSN:0007-0920 ISSN:1532-1827 |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| تدمد: | 1532-1827 0007-0920 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dcd0db048f69f98452dcfbf6d67aecc http://europepmc.org/articles/PMC7374547 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1dcd0db048f69f98452dcfbf6d67aecc |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15321827 00070920 |
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