MEF 2C/miR‐133a‐3p.1 circuit‐stabilizedAQP 1expression maintains endothelial water homeostasis
| العنوان: | |
|---|---|
| المؤلفون: | Rui Ma, Guo‐jie Li, Jian‐hui Cai, Yong Jiang, Ying Zhao, Wen‐long Lin, Sheng‐yu Zhong, Ai‐kun Wang, Xin‐mei Lan |
| المصدر: | FEBS Letters. 593:2566-2573 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Transcription, Genetic, Biophysics, Biochemistry, Umbilical vein, 03 medical and health sciences, Downregulation and upregulation, Cell Movement, Structural Biology, Human Umbilical Vein Endothelial Cells, Genetics, Homeostasis, Humans, MEF2C, Molecular Biology, 030304 developmental biology, Tube formation, 0303 health sciences, Physiological function, Aquaporin 1, Base Sequence, MEF2 Transcription Factors, Chemistry, 030302 biochemistry & molecular biology, Water, Cell Biology, Cell biology, MicroRNAs, Gene Expression Regulation, Mir 133a |
| الوصف: | Aquaporin 1 (AQP1) plays an important role in endothelial functions and is regulated by MEF2C. However, how AQP1 level is stabilized to maintain endothelial water homeostasis is still not clear. Here, we show that AQP1 expression is significantly upregulated by MEF2C transcriptionally and inhibited by miR-133a-3p.1 post-transcriptionally. Meanwhile, MEF2C activates the expression of miR-133a1. Simultaneous overexpression of MEF2C and miR-133a-3p.1 suppresses the aptitude of changes in AQP1 expression caused by either MEF2C or miR-133a-3p.1. Accordingly, the changes in migration and tube formation of human umbilical vein endothelial cells (HUVECs) caused by MEF2C or miR133a-3p.1 are blunted by coexpression of both of them. These data demonstrate that the homeostasis and physiological function of AQP1 in endothelial health are maintained by the MEF2C and miR-133a-3p.1 regulatory circuit. |
| تدمد: | 1873-3468 0014-5793 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dde4698be444054f58495f48c91c672 https://doi.org/10.1002/1873-3468.13516 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1dde4698be444054f58495f48c91c672 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733468 00145793 |
|---|