Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage
| العنوان: | Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage |
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| المؤلفون: | Hoe Saeng Yang, Mi Woon Kim, Howard Kim, Young-Il Jeong |
| المصدر: | Cells, Vol 10, Iss 2190, p 2190 (2021) Cells Volume 10 Issue 9 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Porphyrins, ROS-sensitive, cervical cancer, QH301-705.5, medicine.medical_treatment, Mice, Nude, Uterine Cervical Neoplasms, Photodynamic therapy, Article, Cell Line, HeLa, Mice, Folic Acid, In vivo, Cell Line, Tumor, PEG ratio, medicine, Animals, Humans, Photosensitizer, Particle Size, Biology (General), Mice, Inbred BALB C, Photosensitizing Agents, Chlorophyllides, biology, nanophotosensitizer, Chemistry, Folate Receptors, GPI-Anchored, beta-Cyclodextrins, General Medicine, biology.organism_classification, In vitro, folate receptor, Photochemotherapy, photodynamic therapy, Folate receptor, Biophysics, Nanoparticles, Female, Phototoxicity, Oxidation-Reduction, HeLa Cells |
| الوصف: | The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (H2O2) and particle size distribution changed from monomodal distribution pattern to multimodal pattern. The fluorescence intensity and Ce6 release rate also increased due to the increase in H2O2 concentration, indicating that the nanophotosensitizers displayed ROS sensitivity. The Ce6 uptake ratio, ROS generation and cell cytotoxicity of the nanophotosensitizers were significantly higher than those of the Ce6 itself against HeLa cells in vitro. Furthermore, the nanophotosensitizers showed folate-receptor-specific delivery capacity and phototoxicity. The intracellular delivery of nanophotosensitizers was inhibited by folate receptor blocking, indicating that they have folate-receptor specificity in vitro and in vivo. Nanophotosensitizers showed higher efficiency in inhibition of tumor growth of HeLa cells in vivo compared to Ce6 alone. These results show that nanophotosensitizers of FaPEGbCDseseCe6 conjugates are promising candidates as PDT of cervical cancer. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1de55ec8be8c332545c3b02390a25634 https://www.mdpi.com/2073-4409/10/9/2190 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1de55ec8be8c332545c3b02390a25634 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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