Response to letter regarding article, 'dysregulation of hydrogen sulfide (H2S) producing enzyme cystathionine γ-lyase (CSE) contributes to maternal hypertension and placental abnormalities in preeclampsia'
التفاصيل البيبلوغرافية
العنوان:
Response to letter regarding article, 'dysregulation of hydrogen sulfide (H2S) producing enzyme cystathionine γ-lyase (CSE) contributes to maternal hypertension and placental abnormalities in preeclampsia'
We thank Drs Tsikas and Cooper for their interest in our recent publication on plasma H2S reduction in preeclampsia (Figure 1A1). They questioned the merit of the widely used methylene blue assay to measure plasma H2S levels. We agree that this assay may overestimate the amount of free H2S present in plasma.2 However, the experimental design in our study investigated the relative change in plasma H2S levels between normal pregnancy and preeclampsia. Furthermore, the reduction in plasma H2S in preeclampsia compared with normal pregnancy was accompanied by decreased CSE mRNA and protein expression (Figure 11). Therefore, 3 independent observations (RNA, protein, activity) provide compelling evidence of a net loss of H2S activity in preeclampsia. The cytoprotective pathway of heme oxygenase-1 and cystathionine γ-lyase (CSE), which generate carbon monoxide (CO) and hydrogen sulfide (H2S), respectively, hold promise for preeclampsia therapy …
