FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components
| العنوان: | FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components |
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| المؤلفون: | Ole John Nielsen, Uffe Holmskov, Christine Schoeler Jepsen, Kimmie B Colmorten, Anders Schlosser, Lalit Kumar Dubey, Mark Hammond, Steven P. Templeton, Grith Lykke Sørensen, Jesper B. Moeller |
| المصدر: | Frontiers in Immunology, Vol 9 (2018) Jepsen, C S, Dubey, L K, Colmorten, K B, Moeller, J B, Hammond, M A, Nielsen, O, Schlosser, A, Templeton, S P, Sorensen, G L & Holmskov, U 2018, ' FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components ', Frontiers in Immunology, vol. 9, no. SEP, 1967 . https://doi.org/10.3389/fimmu.2018.01967 Frontiers in Immunology |
| بيانات النشر: | Frontiers Media S.A., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, m-ficolin, receptor, Epithelium, Aspergillus fumigatus, Mice, A549, Immunology and Allergy, complement, immune recognition, Lung, Cells, Cultured, Original Research, Mice, Knockout, biology, Chemistry, Intestinal epithelium, Cell biology, medicine.anatomical_structure, Protein Binding, Human, lcsh:Immunologic diseases. Allergy, 030106 microbiology, Immunology, Receptors, Cell Surface, Respiratory Mucosa, chitin, lung, Cell wall, 03 medical and health sciences, FIBCD1, pulmonary aspergillosis, expression, medicine, Animals, Aspergillosis, Humans, Secretion, Interleukin 8, human, Inflammation, IL-8, pathway, Interleukin-8, Mucin, Mucins, Fungal Polysaccharides, biology.organism_classification, Immunity, Innate, Mice, Inbred C57BL, TLR2, 030104 developmental biology, inflammation, activation, protein, epithelium, lcsh:RC581-607 |
| الوصف: | Aspergillus fumigatus (A. fumigatus) is a ubiquitous fungus of clinical importance associated with development of various pulmonary diseases and allergic hypersensitivity reactions. It is protected against environmental stress by a cell wall that contains polysaccharides such as chitin. We previously demonstrated that fibrinogen C domain-containing protein 1 (FIBCD1) is a membrane-bound protein that binds chitin through a conserved S1 binding site and is expressed in intestinal epithelium and salivary glands. Here, we further localized FIBCD1 protein expression at the surface of bronchial and alveolar human lung epithelium, observed recognition of A. fumigatus cell wall with S1 site-independent recognition. We observed FIBCD1-mediated suppression of IL-8 secretion, mucin production, and transcription of genes associated with airway inflammation and homeostasis in FIBCD1-transfected lung epithelial cells. These modulations were generally enforced by stimulation with A. fumigatus cell wall polysaccharides. In parallel, we demonstrated a FIBCD1-mediated modulation of IL-8 secretion induced by TLR2,−4, and −5. Collectively, our findings support FIBCD1 as a human lung epithelial pattern recognition receptor that recognizes the complex A. fumigatus cell wall polysaccharides and modulates the lung epithelial inflammatory response by suppressing inflammatory mediators and mucins.Aspergillus fumigatus (A. fumigatus) is a ubiquitous fungus of clinical importance associated with development of various pulmonary diseases and allergic hypersensitivity reactions. It is protected against environmental stress by a cell wall that contains polysaccharides such as chitin. We previously demonstrated that fibrinogen C domain-containing protein 1 (FIBCD1) is a membrane-bound protein that binds chitin through a conserved S1 binding site and is expressed in intestinal epithelium and salivary glands. Here, we further localized FIBCD1 protein expression at the surface of bronchial and alveolar human lung epithelium, observed recognition of A. fumigatus cell wall with S1 site-independent recognition. We observed FIBCD1-mediated suppression of IL-8 secretion, mucin production, and transcription of genes associated with airway inflammation and homeostasis in FIBCD1-transfected lung epithelial cells. These modulations were generally enforced by stimulation with A. fumigatus cell wall polysaccharides. In parallel, we demonstrated a FIBCD1-mediated modulation of IL-8 secretion induced by TLR2,−4, and −5. Collectively, our findings support FIBCD1 as a human lung epithelial pattern recognition receptor that recognizes the complex A. fumigatus cell wall polysaccharides and modulates the lung epithelial inflammatory response by suppressing inflammatory mediators and mucins. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-3224 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ecac3cee14c27c153636e8bfce1f477 https://www.frontiersin.org/article/10.3389/fimmu.2018.01967/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1ecac3cee14c27c153636e8bfce1f477 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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