Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
| العنوان: | Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry |
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| المؤلفون: | Mangesh Bhide, Eduard Goffa, Lukáš Plank, Dana Jurkovicova, Božena Smolková, Ľudovít Kulcsár, Pavol Slavik, Michal Mego, Katarína Lešková, Michal Kalman, Peter Bujdak, Lucia Pulzova, Ján Kliment, Jan Roska, Miroslav Chovanec |
| المصدر: | Cancers Cancers, Vol 13, Iss 5573, p 5573 (2021) Volume 13 Issue 21 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer Research, endocrine system diseases, clinical stage I seminoma, Vimentin, macromolecular substances, Filamin, Proteomics, urologic and male genital diseases, Article, Ezrin, proteomics, Rete testis, Biopsy, medicine, RC254-282, mesenchymal type proteins, medicine.diagnostic_test, biology, business.industry, rete testis invasion, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, testicular germ cell tumours, Seminoma, medicine.disease, medicine.anatomical_structure, Oncology, Proteome, Cancer research, biology.protein, business, human activities |
| الوصف: | Simple Summary For clinical stage I (CS I) seminoma patients, management through the risk-adapted strategy with adjuvant carboplatin-based chemotherapy in the presence of risk factors and surveillance in the absence of these factors is the preferred option. In such management, rete testis invasion (RTI) represents a prognostic factor, as its absence, together with a tumour diameter ≤4 cm is associated with a very low relapse risk. To be able to routinely manage CS I seminoma patients through a risk-adapted strategy, reliable biomarkers stratifying the risk of relapse for CS I seminoma patients are urgently required. However, no such biomarker has yet entered routine use in clinical decision-making or clinical guidelines. The lack of consistent prognostic biomarkers for CS I seminoma patients prompted us to compare the proteomic profiles of RTI-positive and -negative CS I seminomas to reveal the molecular mechanism(s) and, in particular, the corresponding biomarkers of RTI invasion. Abstract Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was used to investigate RTI-associated proteins. 64 proteins were differentially expressed in RTI-positive compared to -negative CS I seminomas. Of them, 14-3-3γ, ezrin, filamin A, Parkinsonism-associated deglycase 7 (PARK7), vimentin and vinculin, were validated in CS I seminoma patient cohort. As shown by multivariate analysis controlling for clinical confounders, PARK7 and filamin A expression lowered the risk of RTI, while 14-3-3γ expression increased it. Therefore, we suggest that in real clinical biopsy specimens, the expression level of these proteins may reflect prognosis in CS I seminoma patients. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6694 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ed5aa3c939e17406353c6b100b0d72a http://europepmc.org/articles/PMC8583098 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1ed5aa3c939e17406353c6b100b0d72a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726694 |
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