Thunbergia laurifolia leaf extract partially recovers lead-induced renotoxicity through modulating the cell signaling pathways
| العنوان: | Thunbergia laurifolia leaf extract partially recovers lead-induced renotoxicity through modulating the cell signaling pathways |
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| المؤلفون: | Suksan Changlek, Walla Alelwani, Arwa A. Makki, Naymul Karim, Mohammad Nasiruddin Rana, Md. Atiar Rahman, Jitbanjong Tangpong, Dina Hajjar |
| المصدر: | Saudi Journal of Biological Sciences, Vol 27, Iss 12, Pp 3700-3710 (2020) Saudi Journal of Biological Sciences |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Antioxidant, medicine.medical_treatment, H&E, Hematoxylin-Eosin, Thunbergia laurifolia Linn, TBS, Tris phosphate saline, Renal function, Pharmacology, medicine.disease_cause, 01 natural sciences, DNA, Deoxyribonucleic acid, COX-2, Cyclooxygenase-2, 03 medical and health sciences, ROS, reactive oxygen species, Anti-inflammation, TBARS, Thiobarbituric acid reactive substances, TUNEL, Terminal deoxynucleotidyl transferase dUTP nick end labeling, Pb, lead, medicine, MDA, Malondialdehyde, lcsh:QH301-705.5, ComputingMethodologies_COMPUTERGRAPHICS, Kidney, TNF-α, Tumor necrosis factor-alpha, Chemistry, BW, body weight, Vitamin E, TBST, Tris phosphate buffer saline with Tween 20, ELISA, enzyme-linked immunosorbent assay, GFR, Glomerular filtration rate, iNOS, Inducible nitric oxide synthase, Renotoxicity, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), Oxidative stress, Lead acetate, Apoptosis, Toxicity, TL, Thunbergia laurifolia Linn, Lead (Pb), Original Article, General Agricultural and Biological Sciences, BUN, Blood urea nitrogen, 010606 plant biology & botany |
| الوصف: | Graphical abstract This research investigated the reno-protective effect of Thunbergia laurifolia Linn. (TL) in a lead-induced toxicity test through the modulation of cell signaling pathways. The study carried out to evaluate the effect of TL leaf extracts in Swiss Albino mice exposed to lead acetate (PbAc). Prior to in vivo study, a probable kidney-protective effect of the plant leaf extract was presumed through an activity-specific (PASS) molecular docking analysis. In animal model study, albino mice were divided in seven groups and co-treated with PbAc and TL (100, 200 mg/kgBW) or vitamin E (100 mg/kgBW) for 38 days, whereas the untreated control, TL control, and vehicle control groups received sodium acetate, PbAc, sodium acetate plus mineral oil, respectively. At the end of treatment, blood and kidney tissue were collected for investigating Pb concentration, estimating biochemical profile, evaluating oxidative stress and inflammatory parameters. The histopathological change of kidney along with apoptosis was assessed from kidney sections using H & E staining and TUNEL assay. Pb-exposed mice were found to be increased concentration of Pb in the blood and kidney sample, which further led to increased MDA levels in the plasma, blood, and tissue. Followed by kidney damage, increased expression of TNF-α, iNOS, and COX-2 in kidney tissues were noticed, which were related to elevated TNF-α in the systemic circulation of Pb-treated mice. Co-treatment with TL or vitamin E significantly reduced altered structure and apoptosis of kidney tissues. Downregulation of inflammatory markers especially TNF-α, iNOS, and COX-2 with simultaneous improvement of renal function through reduced plasma BUN and creatinine levels demonstrate that TL act as a potential dietary supplement to detoxify Pb in kidney showing an antioxidant and anti-inflammatory effect. |
| تدمد: | 1319-562X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f3c30bb34dee77d6b905625f0c3774a https://doi.org/10.1016/j.sjbs.2020.08.016 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1f3c30bb34dee77d6b905625f0c3774a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1319562X |
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