Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting
| العنوان: | Skeletogenic Capacity of Human Perivascular Stem Cells Obtained Via Magnetic-Activated Cell Sorting |
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| المؤلفون: | Greg Asatrian, Erin Zou, Aaron W. James, Leslie Chang, Leititia Zhang, Catherine Ding, Bruno Péault, Min Lee, Noah Yan, Kristen P. Broderick, Jiajia Xu, Yiyun Wang, Carolyn A. Meyers |
| المصدر: | Tissue Eng Part A |
| بيانات النشر: | Mary Ann Liebert Inc, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Stromal cell, medicine.medical_treatment, 0206 medical engineering, Population, Biomedical Engineering, Antigens, CD34, Bioengineering, Cell Separation, 02 engineering and technology, Biology, Biochemistry, Biomaterials, 03 medical and health sciences, Osteogenesis, medicine, Humans, Cell Lineage, education, 030304 developmental biology, Wound Healing, 0303 health sciences, education.field_of_study, Magnetic-activated cell sorting, Magnetic Phenomena, Stem Cells, Skull, Mesenchymal stem cell, Cell Differentiation, Original Articles, Stem-cell therapy, Cell sorting, 020601 biomedical engineering, Cell biology, Adipose Tissue, CD146, Stem cell, Biomarkers |
| الوصف: | Human perivascular stem/stromal cells (PSC) are a multipotent mesenchymal progenitor cell population defined by their perivascular residence. PSC are increasingly studied for their application in skeletal regenerative medicine. PSC from subcutaneous white adipose tissue are most commonly isolated via fluorescence-activated cell sorting (FACS), and defined as a bipartite population of CD146(+)CD34(−)CD31(−)CD45(−) pericytes and CD34(+)CD146(−)CD31(−)CD45(−) adventitial cells. FACS poses several challenges for clinical translation, including requirements for facilities, equipment, and personnel. The purpose of this study is to identify if magnetic-activated cell sorting (MACS) is a feasible method to derive PSC, and to determine if MACS-derived PSC are comparable to our previous experience with FACS-derived PSC. In brief, CD146(+) pericytes and CD34(+) adventitial cells were enriched from human lipoaspirate using a multistep column approach. Next, cell identity and purity were analyzed by flow cytometry. In vitro multilineage differentiation studies were performed with MACS-defined PSC subsets. Finally, in vivo application was performed in nonhealing calvarial bone defects in Scid mice. Results showed that human CD146(+) pericytes and CD34(+) adventitial cells may be enriched by MACS, with defined purity, anticipated cell surface marker expression, and capacity for multilineage differentiation. In vivo, MACS-derived PSC induce ossification of bone defects. These data document the feasibility of a MACS approach for the enrichment and application of PSC in the field of tissue engineering and regenerative medicine. IMPACT STATEMENT: Our findings suggest that perivascular stem/stromal cells, and in particular adventitial cells, may be isolated by magnetic-activated cell sorting and applied as an uncultured autologous stem cell therapy in a same-day setting for bone defect repair. |
| تدمد: | 1937-335X 1937-3341 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f41959bf10f35e4af761a157cde86fe https://doi.org/10.1089/ten.tea.2019.0031 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1f41959bf10f35e4af761a157cde86fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1937335X 19373341 |
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