Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rats

التفاصيل البيبلوغرافية
العنوان: Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rats
المؤلفون: Chih-Chien Sung, Brian G. Poll, Shih-Hua Lin, Adrian R. Murillo-de-Ozores, Chung-Lin Chou, Lihe Chen, Chin-Rang Yang, Min-Hsiu Chen, Yu-Juei Hsu, Mark A. Knepper
المصدر: Journal of the American Society of Nephrology. 33:2040-2058
بيانات النشر: Ovid Technologies (Wolters Kluwer Health), 2022.
سنة النشر: 2022
مصطلحات موضوعية: Aquaporin 2, Polyuria, Vasopressins, Nephrology, Animals, RNA, Messenger, General Medicine, Kidney Tubules, Collecting, Kidney, Rats, Ureteral Obstruction
الوصف: Ureteral obstruction is marked by disappearance of the vasopressin-dependent water channel aquaporin-2 (AQP2) in the renal collecting duct and polyuria upon reversal. Most studies of unilateral ureteral obstruction (UUO) models have examined late time points, obscuring the early signals that trigger loss of AQP2.We performed RNA-Seq on microdissected rat cortical collecting ducts (CCDs) to identify early signaling pathways after establishment of UUO.Vasopressin V2 receptor (AVPR2) mRNA was decreased 3 hours after UUO, identifying one cause of AQP2 loss. Collecting duct principal cell differentiation markers were lost, including many not regulated by vasopressin. Immediate early genes in CCDs were widely induced 3 hours after UUO, includingiMyc/i,iAtf3/i, andiFos/i(confirmed at the protein level). Simultaneously, expression of NF-κB signaling response genes known to repressiAqp2/iincreased. RNA-Seq for CCDs at an even earlier time point (30 minutes) showed widespread mRNA loss, indicating a "stunned" profile. Immunocytochemical labeling of markers of mRNA-degrading P-bodies DDX6 and 4E-T indicated an increase in P-body formation within 30 minutes.Immediately after establishment of UUO, collecting ducts manifest a stunned state with broad disappearance of mRNAs. Within 3 hours, there is upregulation of immediate early and inflammatory genes and disappearance of the V2 vasopressin receptor, resulting in loss of AQP2 (confirmed by lipopolysaccharide administration). The inflammatory response seen rapidly after UUO establishment may be relevant to both UUO-induced polyuria and long-term development of fibrosis in UUO kidneys.
تدمد: 1533-3450
1046-6673
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fa560b1e91387740f226ac53d4eaa3c
https://doi.org/10.1681/asn.2022050601
رقم الأكسشن: edsair.doi.dedup.....1fa560b1e91387740f226ac53d4eaa3c
قاعدة البيانات: OpenAIRE