Formation and functionalization of membraneless compartments in Escherichia coli
| العنوان: | Formation and functionalization of membraneless compartments in Escherichia coli |
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| المؤلفون: | Zhi-Gang Qian, Xiao-Xia Xia, Chun-Fei Hu, Fang Pan, Shao-Peng Wei, Meng-Ting Chen, Sang Yup Lee |
| المصدر: | Nature Chemical Biology. 16:1143-1148 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Green Fluorescent Proteins, Intrinsically disordered proteins, medicine.disease_cause, Cell membrane, 03 medical and health sciences, Synthetic biology, Cytosol, Microscopy, Electron, Transmission, Organelle, Escherichia coli, medicine, Molecular Biology, Cellular compartment, 030304 developmental biology, Organelles, 0303 health sciences, Microscopy, Confocal, biology, Chemistry, Cell Membrane, 030302 biochemistry & molecular biology, Gene Expression Regulation, Bacterial, Cell Biology, Dynamic Light Scattering, medicine.anatomical_structure, Microscopy, Fluorescence, Nucleic acid, Biophysics, biology.protein, Fibroins, Resilin |
| الوصف: | Membraneless organelles formed by liquid-liquid phase separation of proteins or nucleic acids are involved in diverse biological processes in eukaryotes. However, such cellular compartments have yet to be discovered or created synthetically in prokaryotes. Here, we report the formation of liquid protein condensates inside the cells of prokaryotic Escherichia coli upon heterologous overexpression of intrinsically disordered proteins such as spider silk and resilin. In vitro reconstitution under conditions that mimic intracellular physiologically crowding environments of E. coli revealed that the condensates are formed via liquid-liquid phase separation. We also show functionalization of these condensates via targeted colocalization of cargo proteins to create functional membraneless compartments able to fluoresce and to catalyze biochemical reactions. The ability to form and functionalize membraneless compartments may serve as a versatile tool to develop artificial organelles with on-demand functions in prokaryotes for applications in synthetic biology. |
| تدمد: | 1552-4469 1552-4450 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ff4a3fc8764df9c46b5e440dc1970ed https://doi.org/10.1038/s41589-020-0579-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....1ff4a3fc8764df9c46b5e440dc1970ed |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15524469 15524450 |
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