A vector-free microfluidic platform for intracellular delivery
| العنوان: | A vector-free microfluidic platform for intracellular delivery |
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| المؤلفون: | Shirley Mao, Nahyun Cho, Armon Sharei, George C. Hartoularos, Klavs F. Jensen, Emily L. Jackson, Jeon Woong Kang, Daniel A. Heller, Woo Young Sim, Janeta Zoldan, Abigail K. R. Lytton-Jean, Daniel G. Anderson, Pamela Basto, Kwang-Soo Kim, Andrea Adamo, Sabine Schneider, Jungmin Lee, Robert Langer, Siddharth Jhunjhunwala, Min-Joon Han |
| المصدر: | Proceedings of the National Academy of Sciences. 110:2082-2087 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cell Membrane Permeability, Induced Pluripotent Stem Cells, Microfluidics, Gene Expression, Nanotechnology, Biology, Diffusion, Mice, Cytosol, Drug Delivery Systems, Animals, Humans, RNA, Small Interfering, Primary cell, Induced pluripotent stem cell, Cell Shape, Cells, Cultured, Multidisciplinary, Nanotubes, Carbon, Electroporation, Proteins, Dendritic Cells, Microfluidic Analytical Techniques, Embryonic stem cell, Biomechanical Phenomena, Drug delivery, Reprogramming, Intracellular, HeLa Cells |
| الوصف: | Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a constriction 30–80% smaller than the cell diameter. The resulting controlled application of compression and shear forces results in the formation of transient holes that enable the diffusion of material from the surrounding buffer into the cytosol. The method has demonstrated the ability to deliver a range of material, such as carbon nanotubes, proteins, and siRNA, to 11 cell types, including embryonic stem cells and immune cells. When used for the delivery of transcription factors, the microfluidic devices produced a 10-fold improvement in colony formation relative to electroporation and cell-penetrating peptides. Indeed, its ability to deliver structurally diverse materials and its applicability to difficult-to-transfect primary cells indicate that this method could potentially enable many research and clinical applications. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ffcc133fc8802c41435a97dc09cfac6 https://doi.org/10.1073/pnas.1218705110 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1ffcc133fc8802c41435a97dc09cfac6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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