Peroxiredoxin 6 phospholipid hydroperoxidase activity in the repair of peroxidized cell membranes
| العنوان: | Peroxiredoxin 6 phospholipid hydroperoxidase activity in the repair of peroxidized cell membranes |
|---|---|
| المؤلفون: | Chandra Dodia, Jian-Qin Tao, Elena M. Sorokina, Sheldon I. Feinstein, Jose P. Vasquez-Medina, Aron B. Fisher |
| المصدر: | Redox Biology Redox Biology, Vol 14, Iss, Pp 41-46 (2018) |
| بيانات النشر: | eScholarship, University of California, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | WT, wild type, 0301 basic medicine, Male, Endothelial cells, Clinical Biochemistry, Histidine mutation, Medical Biochemistry and Metabolomics, GPX4, medicine.disease_cause, Inbred C57BL, Perfused lung, Biochemistry, FOX, ferrous xylenol orange, Lipid peroxidation, chemistry.chemical_compound, Mice, Null cell, 2.1 Biological and endogenous factors, Aetiology, lcsh:QH301-705.5, Lung, Cells, Cultured, Mice, Knockout, Hyperoxia, lcsh:R5-920, Cultured, biology, PHGPx, phospholipid hydroperoxide GSH peroxidase, t-BOOH, tert-butyl hydroperoxide, PCOOH, 1-palmitoyl, 2-linoleoyl, sn-3-glycerophosphocholine hydroperoxide, GPx, GSH peroxidase, Prdx6, peroxiredoxin 6, PMVEC, pulmonary microvascular endothelial cells, Pharmacology and Pharmaceutical Sciences, respiratory system, Recombinant Proteins, Cell Hypoxia, Cell biology, DPPP, diphenylpyrenyl phosphate, medicine.symptom, lcsh:Medicine (General), Research Paper, Cells, Knockout, Phospholipid, 03 medical and health sciences, Phospholipase A2, medicine, Animals, 030102 biochemistry & molecular biology, FA, fatty acid, Organic Chemistry, Cell Membrane, Wild type, Hydrogen Peroxide, Mice, Inbred C57BL, 030104 developmental biology, lcsh:Biology (General), chemistry, PLOOH, phospholipid hydroperoxides, Microvessels, LPCAT, lysophosphatidylcholine acyl transferase, biology.protein, Biochemistry and Cell Biology, Oxidant stress, Oxidative stress, Peroxiredoxin VI |
| الوصف: | Although lipid peroxidation associated with oxidative stress can result in cellular death, sub-lethal lipid peroxidation can gradually resolve with return to the pre-exposure state. We have shown that resolution of lipid peroxidation is greatly delayed in lungs or cells that are null for peroxiredoxin 6 (Prdx6) and that both the phospholipase A2 and the GSH peroxidase activities of Prdx6 are required for a maximal rate of recovery. Like other peroxiredoxins, Prdx6 can reduce H2O2 and short chain hydroperoxides, but in addition can directly reduce phospholipid hydroperoxides. This study evaluated the relative role of these two different peroxidase activities of Prdx6 in the repair of peroxidized cell membranes. The His26 residue in Prdx6 is an important component of the binding site for phospholipids. Thus, we evaluated the lungs from H26A-Prdx6 expressing mice and generated H26A-Prdx6 expressing pulmonary microvascular endothelial cells (PMVEC) by lentiviral infection of Prdx6 null cells to compare with wild type in the repair of lipid peroxidation. Isolated lungs and PMVEC were exposed to tert-butyl hydroperoxide and mice were exposed to hyperoxia (> 95% O2). Assays for lipid peroxidation in wild type control and mutant lungs and cells showed ~4-fold increase at end-exposure. Control lungs and cells showed gradual resolution during a post-exposure recovery period. However, there was no recovery from lipid peroxidation by H26A-Prdx6 lungs or PMVEC. These studies confirm an important role for Prdx6 in recovery from membrane lipid peroxidation and indicate that reduction of H2O2 or short chain hydroperoxides does not play a role in the recovery process. Graphical abstract Conclusion: all 3 enzymatic activities of Prdx6 contribute to the reversal of cell membrane phospholipid peroxidation.fx1 Highlights • Repair of peroxidized lipids did not occur with H26A-Prdx6 Delete semicolons;mutation. • Repair reflects the phospholipid hydroperoxidase and PLA2 activities of Prdx6;Move to next with "bullet mark" "P"eroxidase activity with small hydroperoxides and H2O2 does not play a role in repair. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ffe5b92991484faaa8391a659f83d34 https://escholarship.org/uc/item/50v0279c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....1ffe5b92991484faaa8391a659f83d34 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |