CRISpy-Pop: A Web Tool for Designing CRISPR/Cas9-Driven Genetic Modifications in Diverse Populations
| العنوان: | CRISpy-Pop: A Web Tool for Designing CRISPR/Cas9-Driven Genetic Modifications in Diverse Populations |
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| المؤلفون: | Audrey P. Gasch, Hayley R Stoneman, Trey K. Sato, Russell L. Wrobel, Robert Landick, Michael D. Graham, Matteo De Chiara, Chris Todd Hittinger, David J. Krause, Michael Place, Gianni Liti, Joseph Schacherer |
| المصدر: | G3: Genes, Genomes, Genetics, Vol 10, Iss 11, Pp 4287-4294 (2020) G3: Genes|Genomes|Genetics |
| بيانات النشر: | Oxford University Press, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, population genomics, Population, Computational biology, Investigations, Biology, yeast, QH426-470, 01 natural sciences, Genome, 03 medical and health sciences, Genome editing, crispr/cas9, 010608 biotechnology, Genetics, Humans, CRISPR, genome editing, Clustered Regularly Interspaced Short Palindromic Repeats, Guide RNA, sgrna, education, Molecular Biology, Genetics (clinical), 030304 developmental biology, Gene Editing, 0303 health sciences, education.field_of_study, Genome, Human, Cas9, Human genome, CRISPR-Cas Systems, RNA, Guide, Kinetoplastida, Reference genome |
| الوصف: | CRISPR/Cas9 is a powerful tool for editing genomes, but design decisions are generally made with respect to a single reference genome. With population genomic data becoming available for an increasing number of model organisms, researchers are interested in manipulating multiple strains and lines. CRISpy-pop is a web application that generates and filters guide RNA sequences for CRISPR/Cas9 genome editing for diverse yeast and bacterial strains. The current implementation designs and predicts the activity of guide RNAs against more than 1000 Saccharomyces cerevisiae genomes, including 167 strains frequently used in bioenergy research. Zymomonas mobilis, an increasingly popular bacterial bioenergy research model, is also supported. CRISpy-pop is available as a web application (https://CRISpy-pop.glbrc.org/) with an intuitive graphical user interface. CRISpy-pop also cross-references the human genome to allow users to avoid the selection of guide RNAs with potential biosafety concerns. Additionally, CRISpy-pop predicts the strain coverage of each guide RNA within the supported strain sets, which aids in functional population genetic studies. Finally, we validate how CRISpy-pop can accurately predict the activity of guide RNAs across strains using population genomic data. |
| اللغة: | English |
| تدمد: | 2160-1836 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2057c8473f5a41d51dc409a76440543b http://g3journal.org/lookup/doi/10.1534/g3.120.401498 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2057c8473f5a41d51dc409a76440543b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21601836 |
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