Increased plin2 expression in human skeletal muscle is associated with sarcopenia and muscle weakness
| العنوان: | Increased plin2 expression in human skeletal muscle is associated with sarcopenia and muscle weakness |
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| المؤلفون: | Marco Narici, Laura Barberi, Olivier R. Seynnes, Maria Conte, Stefano Salvioli, Daniela Caporossi, Ermanno Martucci, Miriam Capri, Andrea B. Maier, Antonio Musarò, Elena Bellavista, Alessio Degiovanni, Antonia D’Errico-Grigioni, Giovanni Trisolino, Francesco Vasuri, Aurelia Santoro, Claudio Franceschi |
| المساهمون: | Conte M, Vasuri F, Trisolino G, Bellavista E, Santoro A, Degiovanni Alessio, Martucci E, D'Errico-Grigioni A, Caporossi D, Capri M, Maier AB, Seynnes O, Barberi L, Musarò A, Narici MV, Franceschi C, Salvioli S, Centro Interdipartimentale Galvani (CIG), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Departmento of Experimental, Diagnostic and Specialty Medicine, Institute of Oncology and Transplant Pathology, Reconstructive Hip and Knee Joint Surgery, Istituto Ortopedico Rizzoli, Bologna, Department of Health Science, University of Rome 'Foro Italico', Leiden University Medical Center (LUMC), Norwegian School of Sport Sciences, Norwegian School of Sport Sciences = Norges idrettshøgskole [Oslo] (NIH), Department of Anatomy, Histology, Forensic Medicine and Orthopedic [Roma] (DAHFMO), Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], School of Graduate Entry Medicine and Health, University of Nottingham, UK (UON), Neuromechanics, AMS - Ageing and Morbidity |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 8 (8), pp.e73709. ⟨10.1371/journal.pone.0073709⟩ PLoS ONE; Vol 8 Conte, M, Vasuri, F, Trisolino, G, Bellavista, E, Santoro, A, Degiovanni, A, Martucci, E, D'Errico-Grigioni, A, Caporossi, D, Capri, M, Maier, A B, Seynnes, O, Barberi, L, Musarò, A, Narici, M V, Franceschi, C & Salvioli, S 2013, ' Increased Plin2 Expression in Human Skeletal Muscle Is Associated with Sarcopenia and Muscle Weakness ', PLoS ONE, vol. 8, no. 8, e73709 . https://doi.org/10.1371/journal.pone.0073709 PLoS ONE, Vol 8, Iss 8, p e73709 (2013) PLoS ONE, 8(8):e73709. Public Library of Science PLoS ONE, 8(8) |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Aging, Sarcopenia, protein p53, Mice, 0302 clinical medicine, intramuscular lipid, 80 and over, lcsh:Science, pathophysiology, Aged, 80 and over, 0303 health sciences, clinical article, Muscle Weakness, muscle proteins, STORAGE, Muscle tissue, lipid storage, medicine.medical_specialty, vastus lateralis muscle, Perilipin 2, PERILIPIN 2, complication, EXERCISE, Perilipin-2, animal tissue, lipids, 03 medical and health sciences, FAT INFILTRATION, Humans, human, Muscle Strength, skeletal muscle, muscle thickness, OLDER-ADULTS, protein expression, mouse, Aged, P53, animal model, lcsh:R, Muscle weakness, medicine.disease, muscle aging, Endocrinology, quadriceps femoris muscle, immobilization, CELLS, Perilipin, leg disease, lcsh:Q, 030217 neurology & neurosurgery, skeletal muscles, ACTIVATED RECEPTOR-GAMMA, fat droplet, very elderly, Adipose tissue, lcsh:Medicine, membrane protein, animal, Insulin-Like Growth Factor I, Multidisciplinary, INSULIN SENSITIVITY, article, Skeletal, unclassified drug, adipose tissue, APOPTOSIS, medicine.anatomical_structure, female, perilipin, muscle mass, IGF-1, Muscle, ACTIVATED RECEPTOR-GAMMA, INSULIN SENSITIVITY, FAT INFILTRATION, OLDER-ADULTS, PERILIPIN 2, EXERCISE, APOPTOSIS, STORAGE, CELLS, P53, muscle analysis, Intramuscular fat, perilipin 2, somatomedin C, triacylglycerol, somatomedin C, adipose tissue, adult, aged, aging, animal experiment, controlled study, human tissue, male, muscle function, muscle strength, muscle weakness, nonhuman, sarcopenia, signal transduction, adolescent, metabolism, pathology, young adult, Adolescent, Adult, Animals, Membrane Proteins, Muscle, Skeletal, Signal Transduction, Tumor Suppressor Protein p53, Young Adult, medicine.symptom, Research Article, muscle tissue, Adolescent, Biology, Internal medicine, medicine, biopsy, 030304 developmental biology, Skeletal muscle, biology.protein, physical inactivity, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience; Human aging is associated with a progressive loss of muscle mass and strength and a concomitant fat accumulation in form of inter-muscular adipose tissue, causing skeletal muscle function decline and immobilization. Fat accumulation can also occur as intra-muscular triglycerides (IMTG) deposition in lipid droplets, which are associated with perilipin proteins, such as Perilipin2 (Plin2). It is not known whether Plin2 expression changes with age and if this has consequences on muscle mass and strength. We studied the expression of Plin2 in the vastus lateralis (VL) muscle of both healthy subjects and patients affected by lower limb mobility limitation of different age. We found that Plin2 expression increases with age, this phenomenon being particularly evident in patients. Moreover, Plin2 expression is inversely correlated with quadriceps strength and VL thickness. To investigate the molecular mechanisms underpinning this phenomenon, we focused on IGF-1/p53 network/signalling pathway, involved in muscle physiology. We found that Plin2 expression strongly correlates with increased p53 activation and reduced IGF-1 expression. To confirm these observations made on humans, we studied mice overexpressing muscle-specific IGF-1, which are protected from sarcopenia. These mice resulted almost negative for the expression of Plin2 and p53 at two years of age. We conclude that fat deposition within skeletal muscle in form of Plin2-coated lipid droplets increases with age and is associated with decreased muscle strength and thickness, likely through an IGF-1- and p53-dependent mechanism. The data also suggest that excessive intramuscular fat accumulation could be the initial trigger for p53 activation and consequent loss of muscle mass and strength. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20705b46371a1b28dc3afd0ba9c61a71 http://hdl.handle.net/11585/175075 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....20705b46371a1b28dc3afd0ba9c61a71 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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