Rapid Development of Microsatellite Markers with 454 Pyrosequencing in aVulnerable Fish, the Mottled Skate, Raja pulchra
| العنوان: | Rapid Development of Microsatellite Markers with 454 Pyrosequencing in aVulnerable Fish, the Mottled Skate, Raja pulchra |
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| المؤلفون: | Jung-Ha Kang, Jung-Youn Park, Hyun-Su Jo |
| المصدر: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(13): 6 International Journal of Molecular Sciences Volume 13 Issue 6 Pages 7199-7211 |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Linkage disequilibrium, microsatellite, Locus (genetics), Catalysis, Article, Inorganic Chemistry, Microsatellite Repeat, Raja pulchra, Carcharhiniformes, Animals, Skates, Fish, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Alleles, Genetics, Polymorphism, Genetic, biology, pyrosequencing, Organic Chemistry, High-Throughput Nucleotide Sequencing, General Medicine, biology.organism_classification, Computer Science Applications, Genetic Loci, Genetic structure, Microsatellite, Pyrosequencing, Species richness, Microsatellite Repeats |
| الوصف: | The mottled skate, Raja pulchra, is an economically valuable fish. However, due to a severe population decline, it is listed as a vulnerable species by the International Union for Conservation of Nature. To analyze its genetic structure and diversity, microsatellite markers were developed using 454 pyrosequencing. A total of 17,033 reads containing dinucleotide microsatellite repeat units (mean, 487 base pairs) were identified from 453,549 reads. Among 32 loci containing more than nine repeat units, 20 primer sets (62%) produced strong PCR products, of which 14 were polymorphic. In an analysis of 60 individuals from two R. pulchra populations, the number of alleles per locus ranged from 1–10, and the mean allelic richness was 4.7. No linkage disequilibrium was found between any pair of loci, indicating that the markers were independent. The Hardy–Weinberg equilibrium test showed significant deviation in two of the 28 single-loci after sequential Bonferroni’s correction. Using 11 primer sets, cross-species amplification was demonstrated in nine related species from four families within two classes. Among the 11 loci amplified from three other Rajidae family species three loci were polymorphic. A monomorphic locus was amplified in all three Rajidae family species and the Dasyatidae family. Two Rajidae polymorphic loci amplified monomorphic target DNAs in four species belonging to the Carcharhiniformes class, and another was polymorphic in two Carcharhiniformes species. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20c18ac9b2961d27d083b740c3ba04f2 http://open-repository.kisti.re.kr/cube/handle/open_repository/483918.do |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....20c18ac9b2961d27d083b740c3ba04f2 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |