Eukaryotic initiation factor-2, gamma subunit, suppresses proliferation and regulates the cell cycle via the MAPK/ERK signaling pathway in acute myeloid leukemia
| العنوان: | Eukaryotic initiation factor-2, gamma subunit, suppresses proliferation and regulates the cell cycle via the MAPK/ERK signaling pathway in acute myeloid leukemia |
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| المؤلفون: | Huo Tan, Bo Li, Xiaozhen Zeng, Yimin Chen, Zhenqian Huang, Jielun Lu, Ling Liu, Yawei Zou, Lihua Xu, Shuyi Chen |
| المصدر: | Journal of Cancer Research and Clinical Oncology. 147:3157-3168 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Cancer Research, Cell cycle checkpoint, Carcinogenesis, MAP Kinase Signaling System, Eukaryotic Initiation Factor-2, Cell, Mice, Nude, HL-60 Cells, Biology, Mice, Cell Line, Tumor, medicine, Animals, Humans, RNA, Messenger, Phosphorylation, Cell Proliferation, Mice, Inbred BALB C, Cell Cycle, Myeloid leukemia, General Medicine, Cell cycle, medicine.disease, Up-Regulation, Leukemia, Myeloid, Acute, Leukemia, medicine.anatomical_structure, Oncology, Cell culture, Cancer research, Heterografts, EIF2S3 |
| الوصف: | The expression of eukaryotic translation initiation factor-2 subunit 3 (EIF2S3) in patients with non-small cell lung and colorectal cancer is lower than that in healthy individuals. However, the functions of EIF2S3 remain unclear, and its study in leukemia has not been reported. The article aims to explore the role of EIF2S3 in AML (acute myeloid leukemia) and its underlying mechanism. Reverse transcription-quantitative PCR was performed to evaluate the expression levels of EIF2S3, and its association with patient prognosis was determined. Inducible HEL-EIF2S3 and HL-60-EIF2S3 cell lines were established by retrovirus infection. Cellular proliferation and the cell cycle were analyzed using Cell Counting Kit-8 and flow cytometric analyses. Tumorigenic ability was evaluated using xenograft nude mouse model. Gene expression profiles were analyzed in HL-60-EIF2S3 cells by next-generation sequencing, and WB analysis was performed to detect the expression of related proteins. The expression of EIF2S3 in patients with AML was lower than that experiencing CR (P = 0.02). Furthermore, EIF2S3 overexpression inhibited cellular proliferation, halted G0/1 to S phase cell cycle progression, and inhibited tumorigenicity (P = 0.015). 479 differentially expressed genes were identified between HL60-EIF2S3 DOX (−) and HL60-EIF2S3 DOX ( +) cells via NGS and several of them involved in MAPK/ERK signaling pathway. The phosphorylation levels of ERK decreased when EIF2S3 was overexpressed (P |
| تدمد: | 1432-1335 0171-5216 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20e1cb6f64ef8fa76b5fcee2a121af3b https://doi.org/10.1007/s00432-021-03712-5 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....20e1cb6f64ef8fa76b5fcee2a121af3b |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14321335 01715216 |
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