Accelerated Aging during Chronic Oxidative Stress: A Role for PARP-1
| العنوان: | Accelerated Aging during Chronic Oxidative Stress: A Role for PARP-1 |
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| المؤلفون: | Aalt Bast, Joyce M. J. de Vos-Houben, Gertjan J.M. den Hartog, Leen Timmermans, Geja J. Hageman, Daniëlle M. P. H. J. Boesten |
| المساهمون: | RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, Farmacologie en Toxicologie, Toxicogenomics, RS: CARIM School for Cardiovascular Diseases |
| المصدر: | Oxidative Medicine and Cellular Longevity, Vol 2013 (2013) Oxidative Medicine and Cellular Longevity, 2013:680414. Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity |
| بيانات النشر: | Hindawi Limited, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Aging, Telomerase, Poly Adenosine Diphosphate Ribose, Cell division, Article Subject, Flavonols, Poly (ADP-Ribose) Polymerase-1, Minocycline, Biology, medicine.disease_cause, Biochemistry, chemistry.chemical_compound, Telomere Homeostasis, tert-Butylhydroperoxide, medicine, Humans, lcsh:QH573-671, Cellular Senescence, Flavonoids, lcsh:Cytology, Cell Biology, General Medicine, Hydrogen Peroxide, DNA Methylation, Fibroblasts, Telomere, Molecular biology, Cell biology, Oxidative Stress, chemistry, Chromosomes, Human, Pair 2, DNA methylation, Poly(ADP-ribose) Polymerases, Cell aging, Fisetin, Oxidative stress, HeLa Cells, Research Article |
| الوصف: | Oxidative stress plays a major role in the pathophysiology of chronic inflammatory disease and it has also been linked to accelerated telomere shortening. Telomeres are specialized structures at the ends of linear chromosomes that protect these ends from degradation and fusion. Telomeres shorten with each cell division eventually leading to cellular senescence. Research has shown that poly(ADP-ribose) polymerase-1 (PARP-1) and subtelomeric methylation play a role in telomere stability. We hypothesized that PARP-1 plays a role in accelerated aging in chronic inflammatory diseases due to its role as coactivator of NF-κb and AP-1. Therefore we evaluated the effect of chronic PARP-1 inhibition (by fisetin and minocycline) in human fibroblasts (HF) cultured under normal conditions and under conditions of chronic oxidative stress, induced bytert-butyl hydroperoxide (t-BHP). Results showed that PARP-1 inhibition under normal culturing conditions accelerated the rate of telomere shortening. However, under conditions of chronic oxidative stress, PARP-1 inhibition did not show accelerated telomere shortening. We also observed a strong correlation between telomere length and subtelomeric methylation status of HF cells. We conclude that chronic PARP-1 inhibition appears to be beneficial in conditions of chronic oxidative stress but may be detrimental under relatively normal conditions. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1942-0994 1942-0900 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2135d1aec0977ca4682d9b5046949d0f https://doaj.org/article/8127af93d6ab4da18a134cea60616fcd |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2135d1aec0977ca4682d9b5046949d0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19420994 19420900 |
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