New insights regarding Acinetobacter genomic island-related elements
| العنوان: | New insights regarding Acinetobacter genomic island-related elements |
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| المؤلفون: | Eliane Siebor, Catherine Neuwirth |
| المساهمون: | Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC) |
| المصدر: | International Journal of Antimicrobial Agents International Journal of Antimicrobial Agents, 2020, 56, pp.106117-. ⟨10.1016/j.ijantimicag.2020.106117⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Acinetobacter baumannii, DNA, Bacterial, 0301 basic medicine, Microbiology (medical), Genomic Islands, Klebsiella pneumoniae, [SDV]Life Sciences [q-bio], 030106 microbiology, Enterobacter, medicine.disease_cause, Integron, Integrons, 03 medical and health sciences, 0302 clinical medicine, Drug Resistance, Multiple, Bacterial, Genomic island, Escherichia coli, medicine, Humans, Pharmacology (medical), 030212 general & internal medicine, Proteus mirabilis, Vibrio cholerae, Genetics, biology, Salmonella enterica, Sequence Analysis, DNA, General Medicine, Acinetobacter, biology.organism_classification, Anti-Bacterial Agents, Infectious Diseases, biology.protein |
| الوصف: | The objective of this study was to mobilize the Acinetobacter genomic island 1-A (AGI1-A) from Enterobacter hormaechei EclCSP2185 (E. cloacae complex) and to search for the distribution and structure of AGI1-related elements in the NCBI database. AGI1-A was transferred to Escherichia coli. Analysis of the attachment (att) sites could locate the possible recombination crossover in the att sequences at position 10-11 (GG) in the last 18 bp of trmE. In silico detection of AGI backbones in the wgs database identified AGI variants in Salmonella enterica (83 strains), Vibrio cholerae (33), E. hormaechei (12), Acinetobacter baumannii (2), most belonging to prevalent clones (ST40, ST69, ST114 and ST25 respectively), but also in Escherichia coli (1) and Klebsiella pneumoniae (1). Two groups of backbone were identified: one similar to AGI1, the other with a short segment from a Shewanella element upstream of ORF A022. The MDR regions were inserted by transposition at the res site in four different positions: ATAGG (A. baumannii), CATAG (S. enterica and V. cholerae), TAGGT (S. enterica and K. pneumoniae), and TGCAC (S. enterica) representing four different lineages. In some V. cholerae, E. hormaechei and E. coli, deletion events occurred that eliminated part of the backbone at the left junction. Analysis of the right junction identified a fifth lineage in V. cholerae and E. hormaechei (CCATA). In conclusion, based on the position of the MDR region, AGI-related elements belonged to five groups of closely related genomic islands (AGI1-AGI5), with differences in the backbones that evolved independently over time. |
| اللغة: | English |
| تدمد: | 0924-8579 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22520925fb7235d1cb453711484a5a69 https://hal.archives-ouvertes.fr/hal-03491301 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....22520925fb7235d1cb453711484a5a69 |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 09248579 |
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