PLZF suppresses differentiation of mouse spermatogonial progenitor cells via binding of differentiation associated genes
| العنوان: | PLZF suppresses differentiation of mouse spermatogonial progenitor cells via binding of differentiation associated genes |
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| المؤلفون: | Kang Zou, Min Yuan, Xinglong Shi, Kunying Hao, Jiaxi Liu, Yinjuan Qian, Xiaodong Zhao, Qin Xia, Weixiang Song |
| المصدر: | Journal of Cellular Physiology. 235:3033-3042 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Physiology, Clinical Biochemistry, Kruppel-Like Transcription Factors, Gene Expression, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Animals, Promyelocytic Leukemia Zinc Finger Protein, Luciferase, Binding site, Promoter Regions, Genetic, Gene, Gene knockdown, Adult Germline Stem Cells, Cell Differentiation, Promoter, Cell Biology, Spermatogonia, Up-Regulation, Cell biology, Proto-Oncogene Proteins c-kit, 030104 developmental biology, Real-time polymerase chain reaction, 030220 oncology & carcinogenesis, Chromatin immunoprecipitation |
| الوصف: | Promyelocytic leukaemia zinc finger (PLZF) is a key factor in inhibiting differentiation of spermatogonial progenitor cells (SPCs), but the underlying mechanisms are still largely unknown. In this study, the regulation of PLZF on Kit, Stra8, Sohlh2, and Dmrt1 (SPCs differentiation related genes) was investigated. We found some PLZF potential binding sites existed in the promoters of Kit, Stra8, Sohlh2, and Dmrt1. Additionally, the expressions of KIT, STRA8, SOHLH2, and DMRT1 were upregulated when PLZF was knockdown in SPCs. Furthermore, chromatin immunoprecipitation quantitative polymerase chain reaction revealed PLZF directly bound to the promoters of Kit, Stra8, Sohlh2, and Dmrt1. Besides, dual luciferase assay verified PLZF repressed those gene expressions. Collectively, our finding indicate that PLZF binds to the promoter regions of Kit, Stra8, Sohlh2, and Dmrt1 to regulate SPCs differentiation, which facilitate us to further understand the regulatory mechanism of PLZF in SPCs fates. |
| تدمد: | 1097-4652 0021-9541 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2259199e83678af41672c06a33346e83 https://doi.org/10.1002/jcp.29208 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2259199e83678af41672c06a33346e83 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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