Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR‐558 and regulating TREM1 expression
| العنوان: | Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR‐558 and regulating TREM1 expression |
|---|---|
| المؤلفون: | Xianfa Du, Shunlun Chen, Haitao Cui, Yuming Huang, Jianru Wang, Hui Liu, Zemin Li, Chunxiang Liang, Zhaomin Zheng, Hua Wang |
| المصدر: | Cell Proliferation. 55 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | MicroRNAs, Nucleus Pulposus, Animals, Intervertebral Disc Degeneration, RNA, Circular, Cell Biology, General Medicine, Triggering Receptor Expressed on Myeloid Cells-1, Rats |
| الوصف: | Intervertebral disc degeneration (IVDD) is a leading cause of low back pain. Circular RNAs (circRNAs) have been demonstrated to exert vital functions in IVDD. However, the role and mechanism of hsa_circ_0083756 in the development of IVDD remain unclear.RT-qPCR was performed to detect expressions of hsa_circ_0083756, miR-558 and TREM1 in nucleus pulposus (NP) tissues and cells. CCK8 assay, flow cytometry, TUNEL assay, RT-qPCR and WB were used to clarify the roles of hsa_circ_0083756 in NP cells proliferation and extracellular matrix (ECM) formation. Bioinformatics analyses, dual-luciferase reporter gene experiment, RNA immunoprecipitation (RIP) assay and FISH assay were performed to predict and verify the targeting relationship between hsa_circ_0083756 and miR-558, as well as that between miR-558 and TREM1. Ultimately, the effect of hsa_circ_0083756 on IVDD was tested through anterior disc-puncture IVDD animal model in rats.hsa_circ_0083756 was upregulated in degenerative NP tissues and cells. In vitro loss-of-function and gain-of-function studies suggested that hsa_circ_0083756 knockdown promoted, whereas hsa_circ_0083756 overexpression inhibited NP cells proliferation and ECM formation. Mechanistically, hsa_circ_0083756 acted as a sponge of miR-558 and subsequently promoted the expression of TREM1. Furthermore, in vivo study indicated that silencing of hsa_circ_0083756 could alleviate IVDD in rats.hsa_circ_0083756 promoted IVDD via targeting the miR-558/TREM1 axis, and hsa_circ_0083756 may serve as a potential therapeutic target for the treatment of IVDD. |
| تدمد: | 1365-2184 0960-7722 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::226622c734d54628013d0362174263fa https://doi.org/10.1111/cpr.13205 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....226622c734d54628013d0362174263fa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652184 09607722 |
|---|