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Renal tumouroids: challenges of manufacturing 3D cultures from patient derived primary cells

التفاصيل البيبلوغرافية
العنوان: Renal tumouroids: challenges of manufacturing 3D cultures from patient derived primary cells
المؤلفون: Agata Nyga, Katerina Stamati, Patricia A. Redondo, Tayebeh Azimi, Andrew Feber, Joana B. Neves, Rifat Hamoudi, Nadège Presneau, Soha El Sheikh, Maxine G. B. Tran, Mark Emberton, Marilena Loizidou, Umber Cheema
المصدر: Journal of Cell Communication and Signaling. 16:637-648
بيانات النشر: Springer Science and Business Media LLC, 2022.
سنة النشر: 2022
مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry
الوصف: Recent advancements in 3D in vitro culture have allowed for the development of cancer tissue models which accurately recapitulate the tumour microenvironment. Consequently, there has been increased innovation in therapeutic drug screening. While organoid cultures show great potential, they are limited by the time scale of their growth in vitro and the dependence upon commercial matrices, such as Matrigel, which do not allow for manipulations of their composition or mechanical properties. Here, we show a straightforward approach for the isolation and culture of primary human renal carcinoma cells and matched non-affected kidney. This approach does not require any specific selection for cancer cells, and allows for their direct culture in amenable 3D collagen-based matrices, with the preservation of cancer cells as confirmed by NGS sequencing. This method allows for culture of patient-derived cancer cells in 3D microenvironment, which can be used for downstream experimentation such as investigation of cell–matrix interaction or drug screening.
تدمد: 1873-961X
1873-9601
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22881ab419e13ec646e20bee7c1e8ce8
https://doi.org/10.1007/s12079-022-00666-2
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....22881ab419e13ec646e20bee7c1e8ce8
قاعدة البيانات: OpenAIRE
الوصف
تدمد:1873961X
18739601