Aspergillus flavuswas isolated from the soil field cultivated with wheat crop (Triticum astevium L.), which was treated with Topik EC 100 (Clodinafop-propargyl) herbicide in Al-Fuhud city, southeast of Thi-Qar Governorate-Iraq. soil samples were collected at a depth of (0-15) cm for a period of 17 weeks, and the fungus were isolated from the soil by dilution method, the pH, electrical conductivity, organic matter and soil texture were measured. the results showed thatAspergillus flavuswas dominant in this study, where the percentage of appearance ofAspergillus flavuswas 86%. the fungus was treated with the active ingredient Clodinafop-propargyl of Topik 100 EC herbicide in solid and liquid media (Mineral salt medium). the fungus showed its ability to grow in the medium containing the active substance. the results showed thatAspergillus flavuscould biodegrade the active ingredient. the substances were measured by Fourier Transform Infrared spectroscopy and Gas chromatography- mass spectroscopy, the results showed that biodegradation percentage of the active ingredient Clodinafop-propargyl at a concentration 0.01 ppm byAspergillus flavuswas 80%.
