Exploring Exercise- and Context-Induced Peptide Changes in Mice by Quantitative Mass Spectrometry
| العنوان: | Exploring Exercise- and Context-Induced Peptide Changes in Mice by Quantitative Mass Spectrometry |
|---|---|
| المؤلفون: | Justin S. Rhodes, Martina L. Mustroph, Bruce R. Southey, Elena V. Romanova, Sarah E. Dowd, Heinrich Pinardo, Jonathan V. Sweedler |
| المصدر: | ACS Omega ACS Omega, Vol 3, Iss 10, Pp 13817-13827 (2018) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Chemistry, General Chemical Engineering, Electrospray ionization, Dentate gyrus, Hippocampus, Endogeny, Context (language use), General Chemistry, Extinction (psychology), Amygdala, Conditioned place preference, Article, lcsh:Chemistry, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Endocrinology, medicine.anatomical_structure, lcsh:QD1-999, nervous system, Internal medicine, medicine, 030217 neurology & neurosurgery |
| الوصف: | Recent research suggests that exercise may help facilitate abstinence from cocaine addiction, though the mechanisms are not well understood. In mice, wheel running accelerates the extinction of conditioned place preference (CPP) for cocaine, providing an animal model for evaluating potential neurological mechanisms. The objective of this study was to quantify dynamic changes in endogenous peptides in the amygdala and dentate gyrus of the hippocampus in mice exposed to a context paired with the effects of cocaine, and in response to exercise. Male C57BL/6J mice conditioned to cocaine were housed with or without running wheels for 30 days. Following a CPP test and final exposure to either a cocaine- or saline-associated context, peptides were measured in brain tissue extracts using label-free matrix-assisted laser desorption/ionization mass spectrometry (MS) and stable isotopic labeling with liquid chromatography and electrospray ionization MS. CPP in mice was significantly reduced with running, which correlated to decreased myelin basic protein derivatives in the dentate gyrus extracts, possibly reflecting increased unmyelinated granule neuron density. Exposure to a cocaine-paired context increased hemoglobin-derived peptides in runners and decreased an actin-derived peptide in sedentary animals. These results allowed us to characterize a novel set of biomarkers that are responsive to exercise in the hippocampus and in a cocaine-paired context in the amygdala. |
| تدمد: | 2470-1343 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22dedb31506e30707cd529441f09340b https://pubmed.ncbi.nlm.nih.gov/30411050 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....22dedb31506e30707cd529441f09340b |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 24701343 |
|---|