Single-Cell Microwell Platform Reveals Circulating Neural Cells as a Clinical Indicator for Patients with Blood-Brain Barrier Breakdown
| العنوان: | Single-Cell Microwell Platform Reveals Circulating Neural Cells as a Clinical Indicator for Patients with Blood-Brain Barrier Breakdown |
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| المؤلفون: | Minqiao Zheng, Xianting Ding, Xinlong Huo, Deng Cai, Yanlei Liu, Khan Zara Ahmad, Wenqiong Su, Yu Zhang, Xijun He, Antony R. Warden, Yuqing Ke, Sijia Yan, Hongxia Li, Xiao Zhi |
| المصدر: | Research, Vol 2021 (2021) Research |
| بيانات النشر: | American Association for the Advancement of Science, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, Science, Cell, Central nervous system, Brain damage, Blood–brain barrier, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Nervous system disease, medicine, DAPI, Stroke, Multidisciplinary, biology, business.industry, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, chemistry, biology.protein, NeuN, medicine.symptom, business, 030217 neurology & neurosurgery, Research Article |
| الوصف: | Central nervous system diseases commonly occur with the destruction of the blood-brain barrier. As a primary cause of morbidity and mortality, stroke remains unpredictable and lacks cellular biomarkers that accurately quantify its occurrence and development. Here, we identify NeuN + /CD45 − /DAPI + phenotype nonblood cells in the peripheral blood of mice subjected to middle cerebral artery occlusion (MCAO) and stroke patients. Since NeuN is a specific marker of neural cells, we term these newly identified cells as circulating neural cells (CNCs). We find that the enumeration of CNCs in the blood is significantly associated with the severity of brain damage in MCAO mice ( p < 0.05 ). Meanwhile, the number of CNCs is significantly higher in stroke patients than in negative subjects ( p < 0.0001 ). These findings suggest that the amount of CNCs in circulation may serve as a clinical indicator for the real-time prognosis and progression monitor of the occurrence and development of ischemic stroke and other nervous system disease. |
| اللغة: | English |
| تدمد: | 2639-5274 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23774abd4612160810160abc2303d875 https://doaj.org/article/49286a811ffe467f9f181fe33fd447ea |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....23774abd4612160810160abc2303d875 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26395274 |
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