Golovinomyces cichoracearum effector‐associated nuclear localization of RPW8 .2 amplifies its expression to boost immunity in Arabidopsis
| العنوان: | Golovinomyces cichoracearum effector‐associated nuclear localization of |
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| المؤلفون: | Jing‐Hao Zhao, Yan‐Yan Huang, He Wang, Xue‐Mei Yang, Yan Li, Mei Pu, Shi‐Xin Zhou, Ji‐Wei Zhang, Zhi‐Xue Zhao, Guo‐Bang Li, Beenish Hassan, Xiao‐Hong Hu, Xuewei Chen, Shunyuan Xiao, Xian‐Jun Wu, Jing Fan, Wen‐Ming Wang |
| المصدر: | New Phytologist. 238:367-382 |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Physiology, Plant Science |
| الوصف: | Arabidopsis RESISTANCE TO POWDERY MILDEW 8.2 (RPW8.2) is specifically induced by the powdery mildew (PM) fungus (Golovinomyces cichoracearum) in the infected epidermal cells to activate immunity. However, the mechanism of RPW8.2-induction is not well understood. Here, we identify a G. cichoracearum effector that interacts with RPW8.2, named Gc-RPW8.2 interacting protein 1 (GcR8IP1), by a yeast two-hybrid screen of an Arabidopsis cDNA library. GcR8IP1 physically associated with RPW8.2 with its RING finger domain that is essential and sufficient for the association. GcR8IP1 was secreted and translocated into the nucleus of host cell infected with PM. Association of GcR8IP1 with RPW8.2 led to an increase of RPW8.2 in the nucleus. In turn, the nucleus-localised RPW8.2 promoted the activity of the RPW8.2 promoter, resulting in transcriptional self-amplification of RPW8.2 to boost immunity at infection sites. Additionally, ectopic expression or host-induced gene silencing of GcR8IP1 supported its role as a virulence factor in PM. Altogether, our results reveal a mechanism of RPW8.2-dependent defense strengthening via altered partitioning of RPW8.2 and transcriptional self-amplification triggered by a PM fungal effector, which exemplifies an atypical form of effector-triggered immunity. |
| تدمد: | 1469-8137 0028-646X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::239c960fbc8b67bf3b12e9f17bb5490b https://doi.org/10.1111/nph.18682 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....239c960fbc8b67bf3b12e9f17bb5490b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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