The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: Evolution during disease progression
| العنوان: | The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: Evolution during disease progression |
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| المؤلفون: | Jeffrey I. Gordon, Robert S. Fulton, Chunyan Wang, Holland S. Cordum, Helene Kling-Bäckhed, Jian Xu, Elaine R. Mardis, Lars Engstrand, Lucinda Fulton, Glendoria Elliott, Jennifer Edwards, Marios Giannakis, Jung D. Oh |
| المصدر: | Proceedings of the National Academy of Sciences. 103:9999-10004 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Gastritis, Atrophic, Genotype, Atrophic gastritis, Molecular Sequence Data, Adenocarcinoma, medicine.disease_cause, Genomic Instability, Stomach Neoplasms, medicine, Humans, Base Pairing, Gene, Oligonucleotide Array Sequence Analysis, Genetics, Multidisciplinary, Helicobacter pylori, biology, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Sequence Analysis, DNA, Biological Sciences, Hydrogen-Ion Concentration, Gene signature, biology.organism_classification, medicine.disease, Gene expression profiling, Chronic Disease, Disease Progression, Cancer research, Gastritis, medicine.symptom, Carcinogenesis, Genome, Bacterial |
| الوصف: | Helicobacter pylori produces acute superficial gastritis in nearly all of its human hosts. However, a subset of individuals develops chronic atrophic gastritis (ChAG), a condition characterized in part by diminished numbers of acid-producing parietal cells and increased risk for development of gastric adenocarcinoma. Previously, we used a gnotobiotic transgenic mouse model with an engineered ablation of parietal cells to show that loss of parietal cells provides an opportunity for a H. pylori isolate from a patient with ChAG (HPAG1) to bind to, enter, and persist within gastric stem cells. This finding raises the question of how ChAG influences H. pylori genome evolution, physiology, and tumorigenesis. Here we describe the 1,596,366-bp HPAG1 genome. Custom HPAG1 Affymetrix GeneChips, representing 99.6% of its predicted ORFs, were used for whole-genome genotyping of additional H. pylori ChAG isolates obtained from Swedish patients enrolled in a case-control study of gastric cancer, as well as ChAG- and cancer-associated isolates from an individual who progressed from ChAG to gastric adenocarcinoma. The results reveal a shared gene signature among ChAG strains, as well as genes that may have been lost or gained during progression to adenocarcinoma. Whole-genome transcriptional profiling of HPAG1’s response to acid during in vitro growth indicates that genes encoding components of metal uptake and utilization pathways, outer membrane proteins, and virulence factors are among those associated with H. pylori ’s adaptation to ChAG. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23b5491354950fdc33fbb0a9a92bd78a https://doi.org/10.1073/pnas.0603784103 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....23b5491354950fdc33fbb0a9a92bd78a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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