Xpo7 is a broad-spectrum exportin and a nuclear import receptor
| العنوان: | Xpo7 is a broad-spectrum exportin and a nuclear import receptor |
|---|---|
| المؤلفون: | Markus T. Bohnsack, Tino Pleiner, Katharina Seibel, Christin Kappert, Dirk Görlich, Heinz-Jürgen Dehne, M. Aksu, Henning Urlaub, Samir Karaca |
| المصدر: | Journal of Cell Biology The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Active Transport, Cell Nucleus, Receptors, Cytoplasmic and Nuclear, Importin, macromolecular substances, Biology, Karyopherins, environment and public health, 03 medical and health sciences, XPO1, Xenopus laevis, Report, medicine, Animals, Humans, Nuclear pore, Research Articles, Cell Nucleus, Cell Biology, Transfection, Single-Domain Antibodies, Cell biology, Cell nucleus, 030104 developmental biology, medicine.anatomical_structure, ran GTP-Binding Protein, Cytoplasm, Nuclear Pore, Oocytes, Nuclear transport, Nucleus, Camelids, New World, HeLa Cells |
| الوصف: | Aksu et al. explore the vast cargo spectrum of exportin7/Xpo7 and present anti-Xpo7 nanobodies that acutely inhibit Xpo7’s transport cycles in living cells. Their expression selectively blocks nuclear enrichment of import cargoes as well as nuclear exclusion of export cargoes, establishing Xpo7 as a novel bidirectional nuclear transport receptor. Exportins bind cargo molecules in a RanGTP-dependent manner inside nuclei and transport them through nuclear pores to the cytoplasm. CRM1/Xpo1 is the best-characterized exportin because specific inhibitors such as leptomycin B allow straightforward cargo validations in vivo. The analysis of other exportins lagged far behind, foremost because no such inhibitors had been available for them. In this study, we explored the cargo spectrum of exportin 7/Xpo7 in depth and identified not only ∼200 potential export cargoes but also, surprisingly, ∼30 nuclear import substrates. Moreover, we developed anti-Xpo7 nanobodies that acutely block Xpo7 function when transfected into cultured cells. The inhibition is pathway specific, mislocalizes export cargoes of Xpo7 to the nucleus and import substrates to the cytoplasm, and allowed validation of numerous tested cargo candidates. This establishes Xpo7 as a broad-spectrum bidirectional transporter and paves the way for a much deeper analysis of exportin and importin function in the future. Graphical Abstract |
| وصف الملف: | application/pdf; application/vnd.ms-excel |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23d804b910810bd5ef6c5adc10236921 https://resolver.caltech.edu/CaltechAUTHORS:20180726-140814607 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....23d804b910810bd5ef6c5adc10236921 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |